LZ was bought from Baoji Guokang Bio-Technology Co., Limited, China. Methanol was purchased from Sigma-Aldrich, Germany. Oleic acid, olive oil, sesame oil, and tween 80 were bought from Central Drug House (P) Ltd., New Delhi, India. Sunflower oil, propylene glycol, peppermint oil, and castor oil were bought from Wuhan Senwayer Century Chemical Co., Ltd, China. Corn oil was purchased from Shaanxi Guanjie Technology CO, China. Tween 20 was bought from SCRC, China, while tween 40 was procured from Avondale Lab, England. Tween 60 was purchased from CP, China. Span (20, 60, and 80), PEG (200, 400, and 600), and transcutol P were all bought from Shanghai Ruizheng Chemical Tech Co., Ltd, China.
Tween 80
Manufactured by Central Drug House
Sourced in India
Tween 80 is a non-ionic surfactant commonly used in laboratory settings. It is a polyoxyethylene sorbitan monooleate compound that exhibits emulsifying and solubilizing properties. Tween 80 is utilized to improve the solubility and stability of various substances in aqueous solutions.
Automatically generated - may contain errors
Lab products found in correlation
Sodium dihydrogen phosphate, by Central Drug House (2 mentions)
Chloroform, by Sisco Research Laboratories (2 mentions)
Diethyl ether, by Sisco Research Laboratories (2 mentions)
Carbopol 934, by Central Drug House (2 mentions)
Disodium hydrogen phosphate, by Central Drug House (2 mentions)
Ellman reagent, by HiMedia (2 mentions)
Span 80, by Central Drug House (2 mentions)
Tween20, by Central Drug House (2 mentions)
Stearic acid, by Central Drug House (2 mentions)
Olive oil, by Central Drug House (1 mentions)
Oleic acid, by Central Drug House (1 mentions)
Methanol, by Merck Group (1 mentions)
Sodium chloride (nacl), by Central Drug House (1 mentions)
Potassium dihydrogen phosphate, by Central Drug House (1 mentions)
Ethanol, by Central Drug House (1 mentions)
Potassium chloride (kcl), by Central Drug House (1 mentions)
Hpmc k4m, by Central Drug House (1 mentions)
Hydrochloric acid (hcl), by Central Drug House (1 mentions)
Methanol, by Central Drug House (1 mentions)
Dimethyl sulfoxide (dmso), by Central Drug House (1 mentions)
10 protocols using tween 80
1
Development of Lipid-Based Nano-Formulations
2
Characterization of Pharmaceutical-Grade MA Formulation
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MA (purity > 99.5%) was received as a kind gift sample from Orchid Pharmaceutical Ltd. (Roorkee, India). Hydroxypropyl methylcellulose-K4M (HPMC-K4M), sodium dodecyl sulfate (SLS), polysorbate 80 (Tween ® 80), hydrochloric acid (HCl), methanol (MeOH), dimethyl sulfoxide (DMSO), sodium hydroxide (NaOH), potassium dihydrogen phosphate (KH 2 PO 4 ), sodium chloride (NaCl), ethanol (C 2 H 5 OH), potassium chloride (KCl), disodium hydrogen phosphate dodecahydrate (Na 2 HPO 4 .12H 2 O), N,N-dimethylformamide (DMF), and acetonitrile (CH 3 CN) were commercially obtained from Central Drug House Ltd. (New Delhi, India). The water utilized for all experiments was high-purity/deionized water, which was obtained using a Milli-Q ® water purification unit (Milli-Q Co., Lyon, France). All other materials used were of analytical/pharmaceutical grade.
3
Development and Evaluation of Tetrahydrocurcumin Formulations
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Tetrahydrocurcumin was kindly provided as a gift sample by Sanat Pharmaceuticals Ltd. (New Delhi, India). Carbopol® 934, disodium hydrogen phosphate, sodium dihydrogen phosphate and Tween 80 were purchased from Central Drug House (P) Ltd. (New Delhi, India). Compritol® 888ATO and Phospholipon 90 G were received as a gift sample from Panacea Biotec (Lalru, Punjab, India) and Gattefosse SAS, France, respectively. Chloroform and diethyl ether were purchased from Sisco Research Laboratories (Mumbai, India). Ethylene diamine tetra acetic acid and triethanolamine were provided by SD Fine Chemicals (Mumbai, India). Formalin and hydrochloric acid were supplied by Qualigens Fine Chemicals (Mumbai, India). Ellman reagent and 2,4-dinitro-chlorobenzene were obtained from Himedia Laboratories Ltd. (Mumbai, India). Methanol and polyethylene glycol were obtained from Fisher Scientific (Mumbai, India). 5,6-Carboxyfluoroscein was purchased from Sigma-Aldrich, Co., Burlington, MA, USA. The RayBio® Mouse IL-6 ELISA Kit was provided by Ray Biotech, Inc., Norcross, GA, USA.
4
Formulation and Characterization of Lipid Nanoparticles
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SIM and GLM were procured from Yarrow Chem, Pvt. Ltd India. Tween 80 (T80), and aerosil 200 (A-200) were purchased from Central Drug House (CDH), New Delhi, India. Labra l M1944CS (LMCS) and Transcutol P (TP) were gifted by M/s Gattefosse, Mumbai, India. Capmul MCM (CMCM) was obtained from M/s Abitec Corp., Ohio. Triple distilled water was used throughout the study.
5
Formulation and Characterization of Tramadol HCl Liposomes
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Tramadol HCl was a gift sample from Zydus Cadila Ltd. (Ahmedabad, India). Soya lecithin and cholesterol were gifted by CP Kelco UK Ltd (Surrey, UK). Span20, Span40, Span60, Span80, Tween20, Tween40, Tween60, Tween80 and hydroxypropyl methylcellulose (HPMC) were procured from Central Drug House Pvt. Ltd. (Mumbai, India). Dialysis membrane, membrane filter (0.22 µm), syringe filter (0.22 µm) were purchased from Merck India Ltd. (India). All other chemicals and reagents used in the development of formulations were analytical grade.
6
Clarithromycin Oral Formulation Development
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Clarithromycin was provided by Century Pharmaceuticals Ltd., Vadodara, India. Stearic acid, glyceryl monostearate, Tween 20, Tween 80, Span 80, sodium lauryl sulphate, polyethylene glycol 400 (PEG 400), and propylene glycol were commercially procured from Central Drug House, New Delhi, India. Compritol 888ATO, Gelucire 43/01, Precirol ATO5, Geleol, and Transcutol P were procured from Gattefosse India, Mumbai, India. Monegyl T18 and Softemul® were purchased from Mohini Organics, Mumbai, India. Stearylamine was obtained from Hi-Media, Mumbai, India.
7
Lipid-based Isoniazid Formulation
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Isoniazid was bought from Sigma Aldrich USA, and soy lecithin (Phospholipon ® 90H)
was received as a gift sample from Lipoid GmbH, Germany. Compritol 888 ATO ® was a gift sample from Gattefosse India Pvt Ltd. Stearic acid and Tween 80 were purchased from Central Drug House, Mumbai. All other chemicals and solvents used in the study were of analytical or HPLC grade.
was received as a gift sample from Lipoid GmbH, Germany. Compritol 888 ATO ® was a gift sample from Gattefosse India Pvt Ltd. Stearic acid and Tween 80 were purchased from Central Drug House, Mumbai. All other chemicals and solvents used in the study were of analytical or HPLC grade.
8
Tetrahydrocurcumin-Loaded Topical Delivery
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Tetrahydrocurcumin was a generous gift sample from Sanat Pharmaceuticals Ltd. (New Delhi, India). Carbopol ® 934, disodium hydrogen phosphate, sodium carbonate, sodium dihydrogen phosphate and tween 80 were obtained from Central Drug House (P) Ltd. (New Delhi, India).
Compritol ® 888ATO was obtained as gift sample from Panacea Biotec (Lalru, Punjab).
Chloroform and diethyl ether were purchased from Sisco Research Laboratories (Mumbai, India).
Ethylene diamine tetra acetic acid and triethanolamine were supplied by SD Fine Chemicals (Mumbai, India). Formalin and hydrochloric acid were obtained by Qualigens Fine Chemicals (Mumbai, India). Ellman reagent was purchased from Himedia Laboratories Ltd. (Mumbai, India).
Methanol and polyethylene glycol were obtained from Fisher Scientific (Mumbai, India). Ethanol was obtained from Changshu Yanguan Chemicals (China). Phospholipon G was procured as a gift sample from Gattefosse SAS, France.
Compritol ® 888ATO was obtained as gift sample from Panacea Biotec (Lalru, Punjab).
Chloroform and diethyl ether were purchased from Sisco Research Laboratories (Mumbai, India).
Ethylene diamine tetra acetic acid and triethanolamine were supplied by SD Fine Chemicals (Mumbai, India). Formalin and hydrochloric acid were obtained by Qualigens Fine Chemicals (Mumbai, India). Ellman reagent was purchased from Himedia Laboratories Ltd. (Mumbai, India).
Methanol and polyethylene glycol were obtained from Fisher Scientific (Mumbai, India). Ethanol was obtained from Changshu Yanguan Chemicals (China). Phospholipon G was procured as a gift sample from Gattefosse SAS, France.
9
Silicone and Paraffin Oil Nanoemulsion
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Silicone, paraffin oil, and surfactant (Tween 80) were procured from Central Drug House (New Delhi, India). Silicone oil is a nonpolar solute and has efficient gaseous absorption capacity which makes it suitable oil for our experiment. Paraffin oil is a mixture of C10–C15 alkanes and cycloalkanes which are colorless, antioxidative, and have low viscosity.
The nonionic surfactant Tween 80 (Polyoxyethylene (20) sorbitan monooleate) with the hydrophilic–lipophilic balance of 18, was used for the formulation of silicone and paraffin oil nanoemulsion. The surfactant action is to reduce the interfacial tension between two phases [26 ].
The nonionic surfactant Tween 80 (Polyoxyethylene (20) sorbitan monooleate) with the hydrophilic–lipophilic balance of 18, was used for the formulation of silicone and paraffin oil nanoemulsion. The surfactant action is to reduce the interfacial tension between two phases [26 ].
10
Investigating Anxiolytic Mechanisms of Action
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Diazepam (DZP, Svizzera Healthcare, India) and Buspirone HCl (BSP, Sigma-Aldrich, U.S.A) was used as reference drugs. Flumazenil (FLU, Sigma-Aldrich, U.S.A), a competitive inhibitor of GABAA receptor in the benzodiazepine site and WAY-100635 maleate salt (WAY, Sigma-Aldrich, U.S.A) a 5-HT1A receptor antagonist was used to find out the possible mechanism(s) of action. PNEO was dissolved in 0.01% (v/v) Tween 80 (Central Drug House, India) in saline and treated orally (p.o.). For the intraperitoneal treatments, the reference drugs and other agents were given by dissolving in normal saline (0.9 %w/v NaCl). All samples were freshly prepared before the behavioral testing and treated at 0.1 ml/10 g of body weight (b/w), except Flumazenil, which was treated at 0.2 ml/10 g of b/w.
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