Dulbecco s modified eagle medium nutrient mixture f 12 dmem f12

Trypsin, collagenase II, fetal bovine serum (FBS), dispase II, phosphate buffer solution (PBS, pH 7.2), penicillin (10,000 IU/mL), and streptomycin (10 mg/mL), were obtained from Invitrogen (Waltham, MA). Dulbecco's Modified Eagle Medium/Nutrient Mixture/F‐12 (DMEM/F‐12), Trypan Blue, p‐formaldehyde, poly‐L‐lysine, Triton X‐100, dimethyl sulfoxide (DMSO), propidium iodide (PI), and L‐glutamine were purchased from Sigma‐Aldrich (Saint Louis, MO). Ethylenediaminetetraacetic acid (EDTA) was obtained from Promega (Madison, WI). Purified antibodies, including anti‐neutrophil elastase (NE), anti−α‐SMA, and anti‐human nuclear antigen (HNA), were purchased from Abcam (Cambridge, England). Alexa Fluor 488‐ and Alexa Fluor 594‐conjugated goat anti‐rabbit antibodies and a Qtracker 655 cell‐labeling kit were obtained from LifeTechnologies (Eugene, OR). Flourescein isothiocyanate (FITC)‐conjugated goat anti‐mouse antibody was obtained from Santa Cruz Biotechnology (Dallas, TX). Purified antibody Ki‐67 was purchased from Cell Marque (Rockline, CA). Bovine serum albumin (BSA) was obtained from Calbiochem (San Diego, CA). T75 cell culture flasks and 24‐well plastic plates were purchased from Corning Inc. (Corning, NY). Vectashield mounting medium was purchased from (Vector Laboratories (San Diego, CA). Polymorphoprep was obtained from Axis‐Shield (Oslo, Norway).

α-Syn, Thioflavin T (ThT), 8-anilinonaphthalene-1-sulfonic acid (ANS), Congo red, Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM: F12), fetal bovine serum (FBS), L-glutamine, penicillin, streptomycin, and MTT were purchased from Sigma Co. (St. Louis, MO, USA). CeO₂ NPs, purity: 99.97%, APS: 10–30 nm, SSA: 30–50 m²/g, bulk density: ~0.8–1.1 g/cm³, and true density: 7.132 g/cm³ were purchased from US Research Nanomaterials, Inc. (Houston, USA) and well characterized by different approaches in our previous paper.22 (link)