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Dmi8 inverted tiling microscope

Manufactured by Leica
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The Leica DMi8 is an inverted tiling microscope designed for laboratory applications. It features a modular construction and supports multiple imaging techniques. The DMi8 enables researchers to capture high-resolution images and perform advanced analyses of their samples.

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Lab products found in correlation

Las x lite, by Leica (2 mentions) Las x, by Leica (2 mentions) Ab40879, by Abcam (1 mentions) Ab40873, by Abcam (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) H l alexa fluor 488, by Abcam (1 mentions)

Close spelling variants of this product

DMi8 inverted microscope Tiling microscope DMi8 microscope Inverted microscope

4 protocols using dmi8 inverted tiling microscope

1

Immunofluorescence Staining of Lung Tissue

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Formalin-fixed, paraffin-embedded tissue sections were deparaffinized in xylene, rehydrated, and stained with antiuteroglobin antibody for club cells (1:1,600) (Abcam, ab40873) or antiprosurfactant protein C antibody for alveoli type II (1:1,000) (Abcam, ab40879). Goat anti-rabbit IgG H&L (Alexa Fluor® 488) (Abcam, ab150077) was used as secondary antibody (1:1,000). The nucleus was stained with DAPI (Molecular Probes). Images of three sections per mouse, 100 µm apart, were acquired with Leica DMi8 inverted tiling microscope (Leica Microsystems) and processed using LAS X.
Hariharan V.N., Shin M., Chang C.W., O’Reilly D., Biscans A., Yamada K., Guo Z., Somasundaran M., Tang Q., Monopoli K., Krishnamurthy P.M., Devi G., McHugh N., Cooper D.A., Echeverria D., Cruz J., Chan I.L., Liu P., Lim S.Y., McConnell J., Singh S.P., Hildebrand S., Sousa J., Davis S.M., Kennedy Z., Ferguson C., Godinho B.M., Thillier Y., Caiazzi J., Ly S., Muhuri M., Kelly K., Humphries F., Cousineau A., Parsi K.M., Li Q., Wang Y., Maehr R., Gao G., Korkin D., McDougall W.M., Finberg R.W., Fitzgerald K.A., Wang J.P., Watts J.K, & Khvorova A. (2023). Divalent siRNAs are bioavailable in the lung and efficiently block SARS-CoV-2 infection. Proceedings of the National Academy of Sciences of the United States of America, 120(11), e2219523120.
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2

Biodistribution of Cy3-labeled hsiRNA

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Mice were injected intravenously (tail vein) or subcutaneously (intrascapular) with 10 mg/kg of Cy3-labeled hsiRNA. After 24 hours, mice were sacrificed and tissues were removed, embedded in paraffin, and sliced into 4-μm sections that were mounted on glass slides. Sections were deparaffinized by incubating in Xylene twice for 8 minutes. Sections were rehydrated in serial ethanol dilutions (100%, 95%, and 80%) for 4 minutes each, and then washed twice for 2 minutes with PBS, stained with DAPI. All fluorescent images were acquired with a Leica DMi8 inverted tiling microscope (Leica Microsystems). Images were processed using the Leica software suite (LAS X and LAS X Lite, Leica Microsystems).
Turanov A.A., Lo A., Hassler M.R., Makris A., Ashar-Patel A., Alterman J.F., Coles A.H., Haraszti R.A., Roux L., Godinho B.M., Echeverria D., Pears S., Iliopoulos J., Shanmugalingam R., Ogle R., Zsengeller Z.K., Hennessy A., Karumanchi S.A., Moore M.J, & Khvorova A. (2018). RNAi Modulation of Placental sFLT1 for the Treatment of Preeclampsia.. Nature biotechnology.
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3

Biodistribution of Cy3-labeled hsiRNA

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Mice were injected intravenously (tail vein) or subcutaneously (intrascapular) with 10 mg/kg of Cy3-labeled hsiRNA. After 24 hours, mice were sacrificed and tissues were removed, embedded in paraffin, and sliced into 4-μm sections that were mounted on glass slides. Sections were deparaffinized by incubating in Xylene twice for 8 minutes. Sections were rehydrated in serial ethanol dilutions (100%, 95%, and 80%) for 4 minutes each, and then washed twice for 2 minutes with PBS, stained with DAPI. All fluorescent images were acquired with a Leica DMi8 inverted tiling microscope (Leica Microsystems). Images were processed using the Leica software suite (LAS X and LAS X Lite, Leica Microsystems).
Turanov A.A., Lo A., Hassler M.R., Makris A., Ashar-Patel A., Alterman J.F., Coles A.H., Haraszti R.A., Roux L., Godinho B.M., Echeverria D., Pears S., Iliopoulos J., Shanmugalingam R., Ogle R., Zsengeller Z.K., Hennessy A., Karumanchi S.A., Moore M.J, & Khvorova A. (2018). RNAi Modulation of Placental sFLT1 for the Treatment of Preeclampsia.. Nature biotechnology.
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4

Fluorescence Microscopy Imaging Protocol

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All fluorescent images were acquired with a Leica DMi8 inverted tiling microscope (Leica Microsystems). Images were processed using the Leica software suite (LAS X and LAS X Lite).
Nikan M., Osborn M.F., Coles A.H., Biscans A., Godinho B.M., Haraszti R.A., Sapp E., Echeverria D., DiFiglia M., Aronin N, & Khvorova A. (2017). Synthesis and Evaluation of Parenchymal Retention and Efficacy of a Metabolically Stable, O-phosphocholine-N-docosahexaenoyl-L-serine siRNA Conjugate in Mouse Brain. Bioconjugate chemistry, 28(6), 1758-1766.
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