Pe mouse igg1 isotype control clone mopc 21

The human colorectal cancer cell line HT-29 was obtained from Pasteur Institute of Iran, Tehran. This cell line was cultured in high-glucose Dulbecco’s modified Eagle’s medium (DMEM; Biowest, France) which was supplemented with 10% fetal bovine serum (FBS; Biowest, France) and 1% penicillin-streptomycin (Biowest, France). The cell line was incubated at 37 °C in 5% CO2 atmosphere.
Oxaliplatin and 5-FU were purchased from Mylan S.A.S, France and Ebewe Pharma, Austria, respectively. CINN (Cat. No. C80857) and Rh123 (Cat. No. R8004) were obtained from Sigma Aldrich, Munich, Germany. FITC Mouse Anti-Human CD44 Clone G44–26 (also known as C26) antibody (Cat. No. 560977) from BD Biosciences, San Jose, CA, U.S.A and Human CD133/2 (clone: 293C3) antibody from Miltenyi Biotec, Bergisch Gladbach, Germany were provided. FITC Mouse IgG1, isotype control clone MOPC-21 (Cat. No. 555748; BD Biosciences, San Jose, CA, USA) and PE Mouse IgG1, isotype control clone MOPC-21 (Cat. No. 554680, BD Biosciences, San Jose, CA, USA) served as the control in flow cytometry analysis. Moreover, staining with a non-vital DNA dye such as 7-aminoactinomycin D (7-AAD) (Cat. No. 559763; BD Biosciences, San Jose, CA, U.S.A) and propidium iodide (PI) (Cat. No. P4170; Sigma Aldrich, Munich, Germany) allows for discrimination of dead cells in flow cytometry