THA (≥98%) was purchased from Meilun Biotech Co. Ltd (Dalian, People’s Republic of China). ε-Caprolactone (ε-CL) was purchased from Alfa Aesar (Ward Hill, MA, USA). MPEG (Mn =2,000), stannous octoate [Sn(Oct)2], and MTT were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). Dimethyl sulfoxide (DMSO), methyl alcohol, and acetonitrile (HPLC grade) were supplied by Kelong Co. Ltd. (Chengdu, People’s Republic of China). A TNF-α ELISA kit was purchased from Beijing Cheng Lin Biological Technology Co. Ltd. (Beijing, People’s Republic of China). Ki-67, CD31, and VEGF polyclonal antibody were purchased from Bioworld Technology Co. Ltd. (Nanjing, People’s Republic of China).
Mpeg mn 2000
Manufactured by Merck Group
Sourced in United States
MPEG (Mn 2000) is a polyethylene glycol-based polymer with an average molecular weight of 2000 Daltons. It is a versatile compound used in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Stannous octoate sn oct 2, by Merck Group (2 mentions)
ε caprolactone, by Thermo Fisher Scientific (2 mentions)
Ki 67, by Bioworld Technology (2 mentions)
Antibiotic antimycotic, by Thermo Fisher Scientific (2 mentions)
Trifluoroacetic acid tfa, by Thermo Fisher Scientific (2 mentions)
Dichloromethane dcm n n dimethylformamide dmf, by Avantor (2 mentions)
Trifluoroacetic acid d tfa d, by Thermo Fisher Scientific (2 mentions)
Tetrahydrofuran thf, by Tedia (2 mentions)
Dimethyl sulfoxide (dmso), by Avantor (2 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
L alanine, by Merck Group (1 mentions)
γ h2ax, by Bioworld Technology (1 mentions)
Cd 31 polyclonal antibodies, by Bioworld Technology (1 mentions)
Anti cd31 antibody, by Thermo Fisher Scientific (1 mentions)
Alanine, by Merck Group (1 mentions)
Anti ki67 antibody, by Abcam (1 mentions)
4 protocols using mpeg mn 2000
1
Synthesis and Characterization of THA Loaded PLGA Nanoparticles
2
Synthesis and Characterization of CSPIO
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mPEG (Mn 2000) and l -alanine were purchased from Sigma-Aldrich (St Louis, MO, USA). Trifluoroacetic acid-d (TFA-d) and trifluoroacetic acid (TFA) were purchased from Alfa Aesar (Wall Hill, MA, USA). Ammonium hydroxide solution (28–30%), diethyl ether, toluene, hexane, and ethanol (95%) were purchased from Echo Chemicals (Toufen, Miaoli, Taiwan). Dimethyl sulfoxide (DMSO) was obtained from J.T. Baker (Center Valley, PA, USA). Dulbecco’s modified eagle’s medium (DMEM), fetal bovine serum (FBS), and antimycotic antibiotics were purchased from Gibco (Grand Island, NY, USA). Tetrahydrofuran (THF) was purchased from TEDIA (Fairfield, OH, USA); dichloromethane (DCM), N,N-dimethylformamide (DMF), and chloroform were obtained from AVANTOR (Center Valley, PA, USA) and dried over CaH2 before being used. CSPIO was prepared via a chemical co-precipitation method [30 (link)].
3
Polymer Synthesis and Cellular Assays
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Ola with 99.8% purity was purchased from Meilun Co., Ltd (Dalian, China). MPEG (Mn =2,000) was purchased from Sigma-Aldrich (St Louis, MO, USA), ε-caprolactone from Alfa Aesar (Reston, VA, USA), and stannous octoate (Sn(Oct)2) purchased from Sigma-Aldrich. Dimethylsulfoxide (DMSO), anhydrous ethanol, and methanol (HPLC grade) were purchased from KeLong Co., Ltd (Chengdu, China). γ-H2AX, CC3, Ki-67, and CD-31 polyclonal antibodies were purchased from Bioworld Technology Co. Ltd. (Nanjing, China).
4
Synthesis and Characterization of Chitosan-Coated Superparamagnetic Iron Oxide Nanoparticles
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ʟ-alanine and mPEG (Mn 2000) were purchased from Sigma-Aldrich (St Louis, MO, USA). Trifluoroacetic acid (TFA) and trifluoroacetic acid-d (TFA-d) were purchased from Alfa Aesar (Wall Hill, MA, USA). Ammonium hydroxide solution (28–30%), toluene, hexane, diethyl ether, and 95% ethanol were purchased from Echo Chemicals (Toufen, Miaoli, Taiwan). Dimethyl sulfoxide (DMSO) was purchased from J.T. Baker (Center Valley, PA, USA). Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and antimycotic antibiotics were purchased from Gibco (Grand Island, NY, USA). Tetrahydrofuran (THF) was purchased from TEDIA (Fairfield, OH, USA); dichloromethane (DCM), N,N-dimethylformamide (DMF), and chloroform were obtained from AVANTOR (Center Valley, PA, USA) and dried over CaH2 before being used. Chitosan-coated superparamagnetic iron oxide (CSPIO) was prepared with a chemical co-precipitation method [29 (link)]. Prussian blue and anti-CD31 antibody were purchased from Thermo Fisher (Cheshire, UK). Anti-Ki67 antibody and anti-alpha smooth muscle actin (SMA) antibody were purchased from Abcam (Cambridge, UK).
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