Log In Sign up
The largest database of trusted experimental protocols

Pe cy5 labeled anti cd5 clone 53 7

Manufactured by BioLegend
Visit Supplier

PE-Cy5-labeled anti-CD5 (clone 53–7.3) is a fluorescently-conjugated antibody targeting the CD5 antigen. CD5 is a cell surface glycoprotein expressed on mature T cells and a subset of B cells.

Automatically generated - may contain errors

Lab products found in correlation

Alexa fluor 594 anti vimentin clone epr3776, by Abcam (2 mentions) Apc anti cd31 clone 390, by Thermo Fisher Scientific (2 mentions) Fitc anti ly 6c clone al 21, by BD (2 mentions) Pe cy7 anti gr 1 clone rb6 8c5, by Thermo Fisher Scientific (2 mentions) Apc cy7 anti cd11b clone m1 70, by BioLegend (2 mentions) Pacific blue anti f4 80 clone bm8, by BioLegend (2 mentions) Perm buffer 3, by Thermo Fisher Scientific (2 mentions) Lsr 2, by FlowJo (2 mentions) Anti cd16 32, by BD (2 mentions) Phosflow fix buffer 1, by BD (2 mentions)

Spelling variants of this product

Anti-CD5 (10 citations) CD5-PE (5 citations) Anti-CD5 (clone 53-7.3, (3 citations) CD5 (53-7.3, (3 citations) CD5-PE-Cy5 (2 citations) PE-anti-CD5 (2 citations)

2 protocols using pe cy5 labeled anti cd5 clone 53 7

1

Multiparametric Flow Cytometry of Aortic and Splenic Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single cell suspensions from the aortae and spleen were obtained as described above and incubated with anti-CD16/32 (BD Biosciences, 553142) and stained on ice for 30 minutes with the following antibodies: Alexa Fluor 594-anti-Vimentin (clone EPR3776, Abcam, ab154207), APC-anti-CD31 (clone 390, Invitrogen, 17–0311-80), FITC-anti-Ly-6C (clone AL-21, BD Biosciences, 553104), PE-Cy5-labeled anti-CD5 (clone 53–7.3, BioLegend, 100609), PE-Cy7-anti-Gr-1 (clone RB6–8C5, Invitrogen, 25–5931-81), APC-Cy7-anti-CD11b (clone M1/70, BioLegend, 101225), and Pacific Blue-anti-F4/80 (clone BM8, BioLegend, 123123). For intracellular staining, cells were fixed and permeabilized with buffers (BD Phosflow Fix Buffer I and Perm Buffer III) according to the manufacturer’s instructions, then stained with Alexa Fluor 488-anti-alpha-smooth muscle actin (α-SMA, clone 1A4, eBioscience, 50–112-4644). Cell suspensions were subjected to flow cytometry (Becton Dickinson LSR II) and analyzed using FlowJo10.1.r5. Macrophages were identified as CD11b+/Ly-6Clow/F4/80+ cells. Ly-6Chi monocytes were identified as CD11b+/Ly-6Chi/F4/80low cells. Neutrophils were identified as CD11b+/Gr-hi cells.
Flores A.M., Hosseini-Nassab N., Jarr K.U., Ye J., Zhu X., Wirka R., Koh A.L., Tsantilas P., Wang Y., Nanda V., Kojima Y., Zeng Y., Lotfi M., Sinclair R., Weissman I.L., Ingelsson E., Smith B.R, & Leeper N.J. (2020). Pro-efferocytic nanoparticles are specifically taken up by lesional macrophages and prevent atherosclerosis. Nature nanotechnology, 15(2), 154-161.
+ Open protocol
+ Expand
2

Multiparametric Flow Cytometry of Aortic and Splenic Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single cell suspensions from the aortae and spleen were obtained as described above and incubated with anti-CD16/32 (BD Biosciences, 553142) and stained on ice for 30 minutes with the following antibodies: Alexa Fluor 594-anti-Vimentin (clone EPR3776, Abcam, ab154207), APC-anti-CD31 (clone 390, Invitrogen, 17–0311-80), FITC-anti-Ly-6C (clone AL-21, BD Biosciences, 553104), PE-Cy5-labeled anti-CD5 (clone 53–7.3, BioLegend, 100609), PE-Cy7-anti-Gr-1 (clone RB6–8C5, Invitrogen, 25–5931-81), APC-Cy7-anti-CD11b (clone M1/70, BioLegend, 101225), and Pacific Blue-anti-F4/80 (clone BM8, BioLegend, 123123). For intracellular staining, cells were fixed and permeabilized with buffers (BD Phosflow Fix Buffer I and Perm Buffer III) according to the manufacturer’s instructions, then stained with Alexa Fluor 488-anti-alpha-smooth muscle actin (α-SMA, clone 1A4, eBioscience, 50–112-4644). Cell suspensions were subjected to flow cytometry (Becton Dickinson LSR II) and analyzed using FlowJo10.1.r5. Macrophages were identified as CD11b+/Ly-6Clow/F4/80+ cells. Ly-6Chi monocytes were identified as CD11b+/Ly-6Chi/F4/80low cells. Neutrophils were identified as CD11b+/Gr-hi cells.
Flores A.M., Hosseini-Nassab N., Jarr K.U., Ye J., Zhu X., Wirka R., Koh A.L., Tsantilas P., Wang Y., Nanda V., Kojima Y., Zeng Y., Lotfi M., Sinclair R., Weissman I.L., Ingelsson E., Smith B.R, & Leeper N.J. (2020). Pro-efferocytic nanoparticles are specifically taken up by lesional macrophages and prevent atherosclerosis. Nature nanotechnology, 15(2), 154-161.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!