RPMI-1640 medium, foetal bovine serum, supplements as well as basic laboratory chemicals were purchased from Sigma (Suffolk, UK). Maxisorp™ microtitre plates were obtained from Nunc (Roskilde, Denmark) and Oxley Hughes Ltd (London, UK). Mouse monoclonal antibodies directed against HIF-1α, mTOR and β-actin, as well as rabbit polyclonal Antibodies against phospho-S2448 mTOR, RAGE and HRP-labelled rabbit anti-mouse secondary antibody were purchased from Abcam (Cambridge, UK). Antibodies against phospho-S65 and non-phosphorylated (total) eukaryotic initiation factor 4E binding protein 1 (eIF4E-BP1) were obtained from Cell Signaling Technology (Danvers, MA USA). Goat anti-mouse and goat anti-rabbit fluorescence dye-labelled antibodies were obtained from LI-COR (Lincoln, Nebraska USA). ELISA-based assay kits for the detection of TNFα, IL-1β, SCF and VEGF were purchased from Bio-Techne (R&D Systems, Abingdon, UK). Anti-Tim-3 mouse monoclonal antibody, its single chain variant as well as human Ig-like V-type domain of Tim-3 (amino acid residues 22–124) and human HMGB1 expressed and purified from E. coli (see below for more details) were used in our experiments.11 (link),15 (link) All other chemicals purchased were of the highest grade of purity commercially available.
Goat anti mouse and goat anti rabbit fluorescence dye labelled antibodies
Manufactured by LI COR
Sourced in United States
Goat anti-mouse and goat anti-rabbit fluorescence dye-labelled antibodies are secondary antibodies used for detection in immunoassays and other applications. These antibodies are produced in goats and are conjugated with fluorescent dyes for visualization.
Automatically generated - may contain errors
Lab products found in correlation
Maxisorp microtitre plate, by Thermo Fisher Scientific (3 mentions)
Rpmi 1640 medium, by Merck Group (3 mentions)
Eif4ebp1, by Cell Signaling Technology (2 mentions)
Foetal bovine serum (fbs), by Merck Group (1 mentions)
Rabbit anti mouse secondary antibody, by Abcam (1 mentions)
Antibodies against, by Cell Signaling Technology (1 mentions)
Il 1β, by R&D Systems (1 mentions)
Mouse monoclonal antibody, by Abcam (1 mentions)
Rabbit polyclonal antibody, by Abcam (1 mentions)
Elisa based assay kits for the detection of il 6, by R&D Systems (1 mentions)
Goat anti human ige, by Merck Group (1 mentions)
Vascular endothelial growth factor (vegf), by R&D Systems (1 mentions)
Caffeine, by Merck Group (1 mentions)
Tnf α, by R&D Systems (1 mentions)
Foetal calf serum, by Merck Group (1 mentions)
P aeruginosa lps, by Merck Group (1 mentions)
Rabbit polyclonal antibodies against, by Abcam (1 mentions)
3 protocols using goat anti mouse and goat anti rabbit fluorescence dye labelled antibodies
1
Immunosuppressive Factors in Cancer
2
Cellular Signaling Pathway Analysis
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RPMI-1640 medium, foetal calf serum and supplements, caffeine, bovine liver XOD, PGN, goat anti-human-IgE, caffeine metabolites and an ATP luminometric detection kit were purchased from Sigma (Suffolk, UK). AMP/cAMP detection kits were purchased from Promega (Southampton, UK). Maxisorp™ microtitre plates were obtained from Nunc (Roskilde, Denmark) as well as from Oxley Hughes Ltd (London, UK). Mouse monoclonal antibodies to HIF-1α, mTOR and β-actin as well as rabbit polyclonal antibodies against phospho-S2448 mTOR and phospho-T2446 mTOR were from Abcam (Cambridge, UK). Antibodies against phospho-T389 p70 S6 kinase 1 (p70 S6K1), total and phospho-S65 eukaryotic initiation factor 4E binding protein 1 (eIF4E-BP1) antibodies were obtained from Cell Signalling Technology (Danvers, MA USA). Goat anti-mouse and goat anti-rabbit fluorescence dye-labelled antibodies were obtained from Li-Cor (Lincoln, Nebraska USA). ELISA-based assay kits for the detection of IL-6, TNF-α and VEGF were purchased from R&D Systems (Abingdon, UK). All other chemicals were of the highest grade of purity that were commercially available (from either Sigma or Fisher Scientific).
3
Investigating HIF-1α and mTOR Signaling
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RPMI-1640 medium, foetal calf serum and supplements, P. aeruginosa LPS, were purchased from Sigma (Suffolk, UK). Maxisorp™ microtitre plates were obtained either from Nunc (Roskilde, Denmark) or kindly provided by Oxley Hughes Ltd (London, UK). Mouse monoclonal antibodies to HIF-1α, mTOR and β-actin as well as rabbit polyclonal antibodies against phospho-S2448 mTOR were purchased from Abcam (Cambridge, UK). Antibodies against phospho-T389 p70 S6 kinase 1 (p70 S6K1), total and phospho-S65 eukaryotic initiation factor 4E binding protein 1 (eIF4E-BP1) antibodies were obtained from Cell Signaling Technology (Danvers, MA USA). Goat anti-mouse and goat anti-rabbit fluorescence dye-labelled antibodies were obtained from Li-Cor (Lincoln, Nebraska USA). ELISA-based assay kits for the detection of VEGF, TNF-α and IL-6 were purchased from Bio-Techne (R&D Systems, Abingdon, UK). All other chemicals were of the highest grade of purity and available commercially unless otherwise stated.
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