Headspace SPME-GC/MS was employed to examine the volatile terpenes following our earlier studies [68 (link),69 (link),70 (link)]. Briefly, the 100 mm DVB/CAR/PDMS fused silica fibers (Sigma-Aldrich, St. Louis, MI, USA) were used to absorb the volatiles at 22 °C for 2 h. The volatiles captured by the fibers were subsequently thermally desorbed and analyzed by an Agilent 5975-6890N GC-MS apparatus (Agilent Technologies, Santa Clara, CA, USA) equipped with a HP-1MS fused-silica capillary column. Volatile terpenes were identified by comparing the mass spectra and retention times with those of standard samples or compounds deposited in the NIST 2008 mass spectra library. The relative content of volatile terpenes was quantitatively analyzed according to the standard curve of (E)-β-ocimene.
Agilent 5975 6890n gc ms apparatus
Manufactured by Agilent Technologies
Sourced in United States
The Agilent 5975-6890N GC-MS apparatus is a gas chromatography-mass spectrometry (GC-MS) system designed for the separation, identification, and quantification of chemical compounds. The system consists of a gas chromatograph (GC) coupled with a mass spectrometer (MS) to provide comprehensive analytical capabilities. The GC-MS apparatus is capable of analyzing a wide range of samples, including organic compounds, environmental pollutants, and pharmaceutical materials.
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2 protocols using agilent 5975 6890n gc ms apparatus
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Headspace SPME-GC/MS Analysis of Volatile Terpenes
2
Volatile Profiling of Floral Terpenes
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The volatile analysis was performed following our earlier studies (Gao et al. 2018; (link)Bao et al. 2020; (link)Yang et al. 2022) . Briefly, headspace solid-phase microextraction (HS-SPME) was employed to collect the volatile compounds from flowers of wild Freesia species or transgenic N. benthamiana leaves. Total trapped volatile compounds were thermally desorbed and transferred to an Agilent 5975-6890N GC-MS apparatus (Agilent Technologies) to analyze the compounds which were subsequently identified by comparing mass spectra with the NIST 2008 mass spectra library as well as standard samples. The quantitative calculation of volatile terpenoids depended on the standard curves of linalool, α-terpineol, and β-caryophyllene which were the major volatile terpenes released from wild Freesia species flowers. All the volatile compound detections were performed in triplicate.
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