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Cd51 pe

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The CD51-PE is a flow cytometry reagent that detects the CD51 cell surface marker, also known as the vitronectin receptor. It is conjugated with the fluorescent dye phycoerythrin (PE) for use in immunofluorescence applications.

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Lab products found in correlation

Dextran sulfate sodium (dss), by Merck Group (2 mentions) Cd44 pe, by BD (2 mentions) Cd90 apc, by BD (2 mentions) Cd3 apc, by BioLegend (2 mentions) Cd14 fitc, by BioLegend (2 mentions) Cd105 apc, by BioLegend (2 mentions) Gr1 apc, by Thermo Fisher Scientific (2 mentions) Cd11c fitc, by BioLegend (2 mentions) Cd11b apc, by BioLegend (2 mentions) Caspase 1 p20, by Adipogen (2 mentions) Il 1β, by Cell Signaling Technology (2 mentions) Gsdmdc1, by Santa Cruz Biotechnology (2 mentions) Alpha tubulin, by Proteintech (2 mentions) Nlrp3, by Adipogen (2 mentions) Anti cd45 apc efluor 780, by Thermo Fisher Scientific (2 mentions) Anti cd4 pe cy7, by Thermo Fisher Scientific (2 mentions) Anti cd45.1 fitc, by Thermo Fisher Scientific (2 mentions) Streptavidin apc efluor 780, by Thermo Fisher Scientific (2 mentions) Anti cd31 pe cy7, by Thermo Fisher Scientific (2 mentions) Anti cd150 pe, by BioLegend (2 mentions)

Spelling variants of this product

Pe-anti-CD51 ( (2 citations)

7 protocols using cd51 pe

1

Induction of Pyroptosis and IBD in Mice

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Lipopolysaccharides (LPS) (E. coli O111:B4) and nigericin (14K05-MM) used to induce pyroptosis were purchased from Invitrogen. Dextran sodium sulfate (DSS) (#118K7374V) used to induce inflammatory bowel disease (IBD) mouse model were obtained from Sigma-aldrich. Anti-mouse antibodies like NLRP3 (#AG-20B-0014, Adipogen), Caspase1-p20 (#AG-20B-0042, Adipogen), IL-1β (#12426, Cell Signaling Technology), GSDMDC1 (#sc-393656, Santa Cruz Biotechnology), alpha Tubulin (#66031-1-1 g, Proteintech), HRP goat anti-mouse IgG antibody (#EK010, Zhuangzhibio), and HRP goat anti-rabbit IgG antibody (#EK020, Zhuangzhibio) were used for immunoblot analysis. The anti-mouse antibodies used for flow cytometry analysis were as follows: APC-CD105 (#MJ7/18, Biolegend), APC-CD90 (#OX-7, BD), PE-CD44 (#IM7, BD, USA), PE-CD51 (#RMV-7, eBioscience), APC-CD3 (#17A2, Biolegend), APC-CD45R/B220 (#RA3-6B2, Biolegend), FITC-CD14 (#Sa14-2, Biolegend), FITC-CD11c (#117306, Biolegend), APC-CD11b (#101212, Biolegend) and APC-Gr1 (#17–5931, eBioscience). The IL-1β Enzyme-Linked Immunosorbent Assay (ELISA) kit was purchased from R＆D Systems (#P16807).
Chen Y., Meng W., Ren G., An N., Zhang J., Liu Z., Wu X., Yin W., Hu X., Liu Z., Feng F, & Chen Y. (2023). NLRP3 inflammasome inhibition of OP9 cells enhance therapy for inflammatory bowel disease. Heliyon, 9(7), e18038.
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2

Immunoblot and Immunofluorescence Analysis of NLRP3 Inflammasome

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LPS (E. coli O111:B4) and nigericin (14K05-MM) were from Invitrogen. DSS (#118K7374V) were from Sigma-aldrich. Anti-mouse antibodies used for immunoblot analysis were: IL-1β (#12426, Cell Signaling Technologies), NLRP3 (#AG-20B-0014, Adipogen), Caspase-1 p20 (#AG-20B-0042, Adipogen), alpha Tubulin (#66031-1-1g, Proteintech), Caspase-11 p20 (#AG-20B-0061, Adipogen). Anti-mouse antibodies used for immunofluorescent staining analysis were: GSDMDC1 (#sc-393656, Santa Cruz Biotechnology), FITC Goat Anti-Mouse IgG Antibody (L146A, Gene Copoeia), AF647TM Goat Anti-Mouse IgG Antibody (L125A, Gene Copoeia). Anti-mouse antibodies used for flow analysis were: APC-CD45 (#30-F11, Biolegend), APC-Ter119 (#17-5921, eBioscience), PE-CD44 (#IM7, BD, USA), PE-CD51 (#RMV-7, eBioscience), APC-CD90 (#OX-7, BD), APC-CD105 (#MJ7/18, Biolegend), PE-CD146 (#P1H12, eBioscience), PE-CD166 (#105902, R&D), FITC-Sca-1 (#122505, Biolegend), APC-CD3 (#17A2, Biolegend), FITC-CD11c (#117306, Biolegend), APC-CD11b (#101212, Biolegend), APC-Gr1 (#17-5931, eBioscience), FITC-F4/80 (#123108, Biolegend), APC-CD19 (#17-5921, eBioscience), and FITC-CD14 (#Sa14-2, Biolegend).
Chen Y., Qin X., An Q., Yi J., Feng F., Yin D., An N., Liu Z., Weng L., Chen S., Hu X, & Yin W. (2018). Mesenchymal Stromal Cells Directly Promote Inflammation by Canonical NLRP3 and Non-canonical Caspase-11 Inflammasomes. EBioMedicine, 32, 31-42.
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3

Antibody Panel for Mouse Cell Analysis

Cited 5 times
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The following antibodies were used for flow cytometry: Anti-CD150-PE (TC15–12F12.2; 115904), anti-CD117-PE/Cy7 (2B8; 105814), anti-CD45.2-PE (104; 109807), anti-CD48-Alexa Fluor 647 (HM48–1; 103416) were purchased from BioLegend (San Diego, CA). anti-CD45-APC-eFluor 780 (30-F11; 47–0451-82), anti-Ter119-APC-eFluor 780 (TER-119; 47–5921-82), Streptavidin APC-eFluor 780 (47–4317-82), anti-CD31-PE/Cy7 (MEC13.3; 25–0311-82), anti-CD45.1-FITC (A20; 11–0453-85), anti-B220-APC-eFluor 780 (RA3–6B2; 47–0452-82), anti-CD4-PE/Cy7 (GK1.5; 25–0041-82), anti-CD8a-PE/Cy7 (53–6.7; 25–0081-85), CD51-PE (RMV-7; 13–0512-85), anti-PDGFRα (CD140a)-APC (APA5; 17–1401-81), anti-Ly6A/E(Sca-1)-FITC (D7; 11–5981-82), Anti-CD45-PerCp-Cyanine5.5 (30-F11; 45–0451-82), anti-CD11b (Mac-1)-PE (M1/70; 12–0112-83) were purchased from eBioscience (Thermo Fisher). Anti-lineage panel cocktail (TER-119, RB6–8C5, RA3–6B2, M1/70, 145–2C11 at 1:50 dilution) was from BD Biosciences (559971). Unless otherwise specified, all antibodies were used at a 1:100 dilution. All antibodies were anti-mouse and their specificity was validated in previous studies performed in our laboratory3 (link),8 (link),12 (link),27 (link)–29 (link).
Nakahara F., Borger D.K., Wei Q., Pinho S., Maryanovich M., Zahalka A.H., Suzuki M., Cruz C.D., Wang Z., Xu C., Boulais P.E., Ma'ayan A., Greally J.M, & Frenette P.S. (2019). Engineering a haematopoietic stem cell niche by revitalizing mesenchymal stromal cells. Nature cell biology, 21(5), 560-567.
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4

Antibody Panel for Mouse Cell Analysis

Cited 5 times
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The following antibodies were used for flow cytometry: Anti-CD150-PE (TC15–12F12.2; 115904), anti-CD117-PE/Cy7 (2B8; 105814), anti-CD45.2-PE (104; 109807), anti-CD48-Alexa Fluor 647 (HM48–1; 103416) were purchased from BioLegend (San Diego, CA). anti-CD45-APC-eFluor 780 (30-F11; 47–0451-82), anti-Ter119-APC-eFluor 780 (TER-119; 47–5921-82), Streptavidin APC-eFluor 780 (47–4317-82), anti-CD31-PE/Cy7 (MEC13.3; 25–0311-82), anti-CD45.1-FITC (A20; 11–0453-85), anti-B220-APC-eFluor 780 (RA3–6B2; 47–0452-82), anti-CD4-PE/Cy7 (GK1.5; 25–0041-82), anti-CD8a-PE/Cy7 (53–6.7; 25–0081-85), CD51-PE (RMV-7; 13–0512-85), anti-PDGFRα (CD140a)-APC (APA5; 17–1401-81), anti-Ly6A/E(Sca-1)-FITC (D7; 11–5981-82), Anti-CD45-PerCp-Cyanine5.5 (30-F11; 45–0451-82), anti-CD11b (Mac-1)-PE (M1/70; 12–0112-83) were purchased from eBioscience (Thermo Fisher). Anti-lineage panel cocktail (TER-119, RB6–8C5, RA3–6B2, M1/70, 145–2C11 at 1:50 dilution) was from BD Biosciences (559971). Unless otherwise specified, all antibodies were used at a 1:100 dilution. All antibodies were anti-mouse and their specificity was validated in previous studies performed in our laboratory3 (link),8 (link),12 (link),27 (link)–29 (link).
Nakahara F., Borger D.K., Wei Q., Pinho S., Maryanovich M., Zahalka A.H., Suzuki M., Cruz C.D., Wang Z., Xu C., Boulais P.E., Ma'ayan A., Greally J.M, & Frenette P.S. (2019). Engineering a haematopoietic stem cell niche by revitalizing mesenchymal stromal cells. Nature cell biology, 21(5), 560-567.
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5

Phenotyping Murine and Human Leukemic Cells

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Murine leukemic cells were analyzed by flow cytometry using the following antibodies: CD45.1-APC (BD PharMingen, USA), Lineage cocktail (StemCell Technologies Inc, Canada), Sca-1-PECy7 and c-kit-PE (BD PharMingen). For human leukemic cells, CD45-APC, CD34-BV421, CD90-FITC (BD PharMingen) antibodies were used. For MSC, anti-CD106 (VCAM-1)-biotin, CD51-PE (eBioscience, CA, USA.), CD140a (PDGFRa)-APC, Sca- 1-PE-Cy7 (BD PharMingen) were used.
Lee H.R., Lee G.Y., Kim E.W., Kim H.J., Lee M., Humphries R.K, & Oh I.H. (2021). Reversible switching of leukemic cells to a drugresistant, stem-like subset via IL-4-mediated cross-talk with mesenchymal stroma. Haematologica, 107(2), 381-392.
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6

Multiparametric Flow Cytometry Analysis of Musculoskeletal Cell Types

Cited 1 time
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Single cell suspensions were stained with fluorochrome-conjugated monoclonal antibodies and a 2.4G2 blocking antibody in the dark for 30 min on ice. Samples were run on a FACSAria III (BD Biosciences), and analyzed using FlowJo (Tree Star Inc). Maintenance of YFP expression during intranuclear staining was performed as previously described28 (link). The antibodies listed below were used for flow cytometry: CD4-eF450 (Clone: GK1.5, eBioscience), CD29-APC-eF780 (Clone: BioHMb1-1, Invitrogen), CD45-BV570 (Clone: 30-f11, Biolegend), CD49f-PerCP-eF710 (Clone: BioGoH3, Invitrogen), CD51-PE (Clone: RMV-7, eBioscience), CD105-APC (Clone: MJ7/18, eBioscience), CD105-eF450 (Clone: MJ7/18, eBioscience), CD200-PerCP-eF710 (Clone: OX90, ebioscience), CD202b-AF647 (Clone: TEK, Biolegend), Lineage (CD3, B220, CD11b, GR-1, TER11-9)-PerCP-Cy5.5 (BD Biosciences), TER119-SuperBright645 (Clone: TER119, Invitrogen), Thy1.2 (CD90.2)-AF700 (Clone: 30-H12, Biolegend), and 6C3-Pe-Cy7 (Clone: 6C3, Biolegend). The following cellular subsets were sorted on the FACSAria III (BD Biosciences): splenic T cells (CD45+TCRβ+), splenic B cells (CD45+CD19+B220+), end bone chondrocytes (CD45−Lin−), end bone lymphocytes (CD45+Lin+), wrist chondrocytes (CD45−Lin−), wrist lymphocytes (CD45+Lin+).
McNamara J.T., Huntington K.E., Borys S., Jayasuriya C.T, & Brossay L. (2021). SHP-2 deletion in CD4Cre expressing chondrocyte precursors leads to tumor development with wrist tropism. Scientific Reports, 11, 20006.
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7

Comprehensive Flow Cytometry Analysis

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Single cell suspensions were stained with fluorochrome-conjugated monoclonal antibodies and a 2.4G2 blocking antibody in the dark for 30 minutes on ice. Samples were run on a FACSAria III (BD Biosciences), and analyzed using FlowJo (Tree Star Inc). Maintenance of YFP expression during intranuclear staining was performed as previously described 28 (link) . The antibodies listed below were used for flow cytometry: CD4-eF450 (Clone: GK1.5, eBioscience), CD29-APC-eF780 (Clone: BioHMb1-1, Invitrogen), CD45-BV570 (Clone: 30-f11, Biolegend), CD49f-PerCP-eF710 (Clone: BioGoH3, Invitrogen), CD51-PE (Clone: RMV-7, eBioscience), CD105-APC (Clone: MJ7/18, eBioscience), CD105-eF450 (Clone: MJ7/18, eBioscience), CD200-PerCP-eF710 (Clone: OX90, ebioscience), CD202b-AF647 (Clone: TEK, Biolegend), Lineage (CD3, B220, CD11b, GR-1, CDC11c, TER11-9) -PerCP-Cy5.5 (BD Biosciences), TER119-SuperBright645 (Clone: TER119, Invitrogen), Thy1.2 (CD90.2)-AF700 (Clone: 30-H12, Biolegend), and 6C3-Pe-Cy7 (Clone: 6C3, Biolegend).
Brossay L., McNamara J., Huntington K.E., Borys S., & Jayasuriya C.T. (2021). SHP-2 deletion in CD4+ chondrocyte precursors leads to tumor development with wrist tropism.
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