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Eclipse ti series confocal microscope

Manufactured by Nikon
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The ECLIPSE Ti Series Confocal Microscope is a high-performance laboratory equipment designed for advanced imaging applications. It features a modular design, allowing for customization to meet specific research requirements. The microscope utilizes laser-based confocal technology to provide high-resolution, three-dimensional images of samples with exceptional clarity and detail.

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Lab products found in correlation

Trueblack lipofuscin autofluorescence quencher, by Merck Group (2 mentions) Bz x800 fluorescence microscope, by Nikon (2 mentions) Dapi mounting media, by Merck Group (2 mentions)

2 protocols using eclipse ti series confocal microscope

1

Multimodal Lung Tissue Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Yale pathology kindly provided assistance with embedding, sectioning of lung tissue. A pulmonary pathologist reviewed the slides blinded and identified immune cell infiltration and other related pathologies. Paraffin embedded lung tissue (fixed in 4% paraformaldehyde for no more than 24 hours) sections were deparaffinized in xylene and rehydrated. After antigen retrieval with 10 mM Sodium Citrate pH 6 and permeabilization with 0.1% Triton-X for 10 min the slides were blocked with 5% BSA in PBS with 0.05% Tween 20 for an hour. Then the samples were stained with primary antibodies against SARS-CoV-2-dsRNA; SARS-CoV2-RNA-dependent RNA Polymerase, SARS-CoV-2-Spike, human CD68, human ACE2 their isotype controls diluted in 1%BSA overnight at 2–8 °C. The next day, the samples were washed and incubated with fluorescent secondary antibodies. After washes, samples were treated with TrueBlack lipofuscin autofluorescence quencher for 30 seconds and mounted on DAPI mounting media (Sigma). Images were acquired using Keyence BZ-X800 Fluorescence Microscope or Nikon ECLIPSE Ti Series Confocal Microscope. Pseudo-colors were assigned for visualization.
Sefik E., Qu R., Junqueira C., Kaffe E., Mirza H., Zhao J., Brewer J.R., Han A., Steach H.R., Israelow B., Blackburn H.N., Velazquez S.E., Chen Y.G., Halene S., Iwasaki A., Meffre E., Nussenzweig M., Lieberman J., Wilen C.B., Kluger Y, & Flavell R.A. (2022). Inflammasome activation in infected macrophages drives COVID-19 pathology. Nature, 606(7914), 585-593.
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2

Multimodal Lung Tissue Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Yale pathology kindly provided assistance with embedding, sectioning of lung tissue. A pulmonary pathologist reviewed the slides blinded and identified immune cell infiltration and other related pathologies. Paraffin embedded lung tissue (fixed in 4% paraformaldehyde for no more than 24 hours) sections were deparaffinized in xylene and rehydrated. After antigen retrieval with 10 mM Sodium Citrate pH 6 and permeabilization with 0.1% Triton-X for 10 min the slides were blocked with 5% BSA in PBS with 0.05% Tween 20 for an hour. Then the samples were stained with primary antibodies against SARS-CoV-2-dsRNA; SARS-CoV2-RNA-dependent RNA Polymerase, SARS-CoV-2-Spike, human CD68, human ACE2 their isotype controls diluted in 1%BSA overnight at 2–8 °C. The next day, the samples were washed and incubated with fluorescent secondary antibodies. After washes, samples were treated with TrueBlack lipofuscin autofluorescence quencher for 30 seconds and mounted on DAPI mounting media (Sigma). Images were acquired using Keyence BZ-X800 Fluorescence Microscope or Nikon ECLIPSE Ti Series Confocal Microscope. Pseudo-colors were assigned for visualization.
Sefik E., Qu R., Junqueira C., Kaffe E., Mirza H., Zhao J., Brewer J.R., Han A., Steach H.R., Israelow B., Blackburn H.N., Velazquez S.E., Chen Y.G., Halene S., Iwasaki A., Meffre E., Nussenzweig M., Lieberman J., Wilen C.B., Kluger Y, & Flavell R.A. (2022). Inflammasome activation in infected macrophages drives COVID-19 pathology. Nature, 606(7914), 585-593.
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