Pr sc 7208

Manufactured by Santa Cruz Biotechnology

Sourced in United States

The PR (sc-7208) is a laboratory equipment product from Santa Cruz Biotechnology. It serves as a core function within the laboratory setting.

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Sc-7208 (11 citations) Anti-PR (H-190) (sc-7208) (3 citations)

6 protocols using pr sc 7208

Protein Expression Analysis in Breast Cancer

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Protein was extracted from breast cancer tissues, MCF7 cells, and MDA-MB-231 cells by homogenization with T-PER buffer. Protein samples were loaded in equal amounts to SDS-PAGE gels and were proceeded to electrophoresis. Gels were blotted to PVDF membranes, which were blocked and incubated with primary antibodies. After overnight incubation, the membranes were washed and incubated with secondary antibodies (211-032-171 anti-rabbit, Jackson laboratory; bs-0296G-HRP anti-mouse, Bioss). Bands were observed with ECL solution (XLS025-0000, Cyanagen) after washing three times.
The following primary polyclonal antibodies were used: rabbit anti-β-actin (sc-130656, Santa Cruz Biotechnology), PR (sc-7208, Santa Cruz), HER2 (4290, Cell Signaling Technology, CST), PCNA (13110, CST), phosphor-AKT (4060, CST), phosphor-ERK (9789, CST), PARP (9532, CST), FAK (13430, CST), rabbit monoclonal antibody to PGRMC1 (13856, CST), and mouse monoclonal antibody to ERα (sc-71064, Santa Cruz Biotechnology).

Lee S.R., Lee Y.H., Jo S.L., Heo J.H., Kim G., Lee G.S., An B.S., Baek I.J, & Hong E.J. (2021). Absence of progesterone receptor membrane component 1 reduces migration and metastasis of breast cancer. Cell Communication and Signaling : CCS, 19, 42.

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Comprehensive Western Blot Antibody Panel

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This assay was performed as described previously [14 ]. Primary antibodies used in this study are: ER (6F11) from Abcam, Cambridge, MA, USA; PR (sc-7208) and BCL2 (sc-509) from Santa Cruz Biotechnology, Santa Cruz, CA, USA; P-AKT-Thr308 (#2214) and P-AKT-Ser473 (#2118) from Epitomics, Burlingame, CA, USA; P-PRAS40-Thr246 (#2997), P-GSK3-Ser9 (#9323), P-ERK1/2-Thr202/Tyr204 (#9101), P-S6-Ser240/244 (#2211), P-4EBP1-Thr37/46 (#9459), P-mTOR-Ser2448 (#2971), AKT (#9272), ERK1/2 (#9102), GSK-3β (#9832), PTEN (#9559), β-actin (#4970), and c-PARP (#9541) from Cell Signaling Technology, Danvers, MA, USA. All our shown Western blotting images are from the same gel with the same exposure to allow for a complete comparison between lines and across treatments.

Fu X., Creighton C.J., Biswal N.C., Kumar V., Shea M., Herrera S., Contreras A., Gutierrez C., Wang T., Nanda S., Giuliano M., Morrison G., Nardone A., Karlin K.L., Westbrook T.F., Heiser L.M., Anur P., Spellman P., Guichard S.M., Smith P.D., Davies B.R., Klinowska T., Lee A.V., Mills G.B., Rimawi M.F., Hilsenbeck S.G., Gray J.W., Joshi A., Osborne C.K, & Schiff R. (2014). Overcoming endocrine resistance due to reduced PTEN levels in estrogen receptor-positive breast cancer by co-targeting mammalian target of rapamycin, protein kinase B, or mitogen-activated protein kinase kinase. Breast Cancer Research : BCR, 16, 430.

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Western Blot Analysis of ESR1, PR, and p53

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Whole cell lysate were collected using methods previous described (24 (link)). Proteins were separated using a 10% Bis-Tris gel and transferred to an immobilon-P membrane (Millipore). Primary antibodies used included ESR1 (sc-542) (1:1000), PR (sc-7208) (1:1000), and p53 (sc-6243) (1:1000) (Santa Cruz Biotechnology) and signals were detected using the Peirce ECL 2 Western Blotting Substrate (Thermo Scientific).

Ren Y.A., Mullany L.K., Liu Z., Herron A.J., Wong K.K, & Richards J.S. (2016). Mutant p53 promotes epithelial ovarian cancer by regulating tumor differentiation, metastasis, and responsiveness to steroid hormones. Cancer research, 76(8), 2206-2218.

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Breast Cancer Signaling Pathway Assay

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Mifepristone (MFP), 17β-estradiol (E2), and 4-hydroxytamoxifen (TAM) were purchased from Sigma-Aldrich. Telapristone acetate (TLP) is from Repros Therapeutics (The Woodlands). FGF2 (sc-79), total AKT (sc-8312), ERK (sc-94), pERK (sc-7383), PKCα (sc-208), PR (sc-7208), and ERα (sc-543) were all purchased from Santa Cruz Biotechnology, and phosphorylated Ser473 AKT (4060) was purchased from Cell Signaling Technology. Secondary antibodies for Western blot and immunohistochemistry were obtained from Amersham Pharmacia Biotech and Vector Laboratories, respectively.

Sahores A., Figueroa V., May M., Liguori M., Rubstein A., Fuentes C., Jacobsen B.M., Elía A., Rojas P., Sequeira G.R., Álvarez M.M., González P., Gass H., Hewitt S., Molinolo A., Lanari C, & Lamb C.A. (2018). Increased High Molecular Weight FGF2 in Endocrine-Resistant Breast Cancer. Hormones & Cancer, 9(5), 338-348.

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Chromatin Immunoprecipitation (ChIP) Assay

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Chromatin immunoprecipitation (ChIP) experiments were performed using the ChIP-IT Express Kit (Active Motif) according to the manufacturer’s instructions. Isolated chromatin was sheared using sonication and sheared chromatin was IP’d overnight using the following primary antibodies (or species-specific IgG control): PR (SC-7208; Santa Cruz Biotechnology), PR (MS-298-P-A; ThermoFisher), and phospho-STAT5 (ab30648; Abcam). IP’d chromatin was analyzed using qRT-PCR with primers designed to amplify select gene promoters/enhancers. Data shown as percentage IP’d DNA over input DNA. ChIP-qPCR primer sequences are listed in Supplementary Table 1.

Holloran S.M., Nosirov B., Walter K.R., Trinca G.M., Lai Z., Jin V.X, & Hagan C.R. (2020). Reciprocal fine-tuning of progesterone and prolactin-regulated gene expression in breast cancer cells. Molecular and cellular endocrinology, 511, 110859.

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Western Blot Analysis of PR, STAT5, PRLR

Cited 2 times

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Western blot analysis was performed as previously described [24 (link), 30 (link)]. Primary antibodies for immunoblotting are as follows: PR (sc-7208; Santa Cruz Biotechnology), STAT5A (ab32043; Abcam), STAT5B (1656; Santa Cruz Biotechnology), Prolactin Receptor (13552; Cell Signaling), and beta-tubulin (2128; Cell Signaling).

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