Bortezomib

Manufactured by MedChemExpress

Sourced in United States

Bortezomib is a proteasome inhibitor used in research laboratories. It is a white to off-white powder that is soluble in organic solvents. Bortezomib functions by inhibiting the 26S proteasome, a complex involved in the degradation of proteins within cells.

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Lab products found in correlation

13 protocols using bortezomib

NSG Xenograft Tumor Study

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Animal experiments were performed under Harvard Institutional Animal Care and Use Committee protocol IS00001059 following ethical approval and NIH guidelines for animal welfare were followed. Mice were housed in 50% humidity at 70°F ambient temperature and exposed to room lighting from 7 am to 7 pm. Animals were housed and cared for according to standard guidelines with free access to water and food. All experiments were performed on 8-16 week old NOD-scid IL2Rgamma^null NSG mice (Jackson labs stock#005557) of both sexes. Animals were randomly assigned to experimental groups. Mice were injected with 10⁶ cells in matrigel subcutaneously. Mice were monitored daily for tumor growth and treatment was initiated at a tumor volume of 150 mm³, with treatment subsequently lasting 4 weeks. Venetoclax cohort of mice were treated by oral gavage daily with ABT-199 (Venetoclax) (Medchem express) at a dose of 100 mg/kg in a mixture of 60% phosal 50 PG, 30% PEG 400, and 10% EtOH. Bortezomib cohort were treated every other day by IP Bortezomib (Medchem express) injection at a dose of 0.8 mg/kg in 0.9% saline. Vehicle treated mice received both vehicles. Tumor growth was monitored every other day.

Fraser C.S., Spetz J.K., Qin X., Presser A., Choiniere J., Li C., Yu S., Blevins F., Hata A.N., Miller J.W., Bradshaw G.A., Kalocsay M., Sanchorawala V., Sarosiek S, & Sarosiek K.A. (2022). Exploiting endogenous and therapy-induced apoptotic vulnerabilities in immunoglobulin light chain amyloidosis with BH3 mimetics. Nature Communications, 13, 5789.

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Flow Cytometric Analysis of ALCAM/CD166

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Anti-ALCAM/CD166 (#343905) antibody for flow cytometry analysis was ordered from Biolegend Inc. Recombinant proteins, including human ALCAM-Fc (#CD6-H5259) and EGF (#10605-HNAE), were ordered from Acrobiosystems and Sinobiology Inc., respectively. Anti-EGF (#AF236) and anti-CD6 (#AF627) neutralizing antibodies were ordered from R&D Systems Inc. EGFR inhibitor gefitinib (#S1025), SMO inhibitor cyclopamine (#S1146), and Gli1 inhibitor GANT61 (#S8075) were ordered from Selleck Inc. Two mitochondrial respiratory chain inhibitors—oligomycin (#C3007) and rotenone (#B5462)—were ordered from ApexBio Technology. Melphalan (#148-82-3) and bortezomib (#179324-69-7) were ordered from MedChemExpress.

Wang F., Dan Z., Luo H., Huang J., Cui Y., Ding H., Xu J., Lin Z., Gao Y., Zhai X., Yang Y., Qu Y., Zhang L., Chen F., Wang Q., Wang X., Feng Y., Liu T., Yi Q., Niu T, & Zheng Y. (2022). ALCAM regulates multiple myeloma chemoresistant side population. Cell Death & Disease, 13(2), 136.

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Comprehensive Protocol for Cell Stress Assays

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JNUTS013 (≥98% purity) was synthesized in the Tang laboratory. Lipopolysaccharide (LPS, from Escherichia coli O111:B4) was purchased from Sigma-Aldrich (Cat#L2630, Steinheim, Germany). Dimethyl sulfoxide (DMSO) was purchased from Aladdin Bio-Chem Technology (Cat#67–68-5, Shanghai, China). d-galactosamine (D-galactosamine) was purchased from Sigma-Aldrich (Cat#G1639, Steinheim, Germany). Hydrocortisone (Cat#HY-N0583), curcumin (Cat#HY-N0005), rapamycin (Cat#HY-10219), MG132 (Cat#HY-13259), bortezomib (Cat#HY-10227), hydroxychloroquine (Cat#HY-W031727), leupeptin hemisulfate (Cat#HY-18234A), bafilomycin A1 (Cat#HY-100558), 3-methyladenine (Cat#HY-19312), dextran sulfate sodium salt (DSSS, Cat#HY-116282B) and disuccinimidyl suberate (DSS) (Cat#HY-W019543) were purchased from MedChemExpress (Monmouth Junction, NJ, USA). Phorbol 12-myristate 13-acetate (PMA, Cat#S7791), nigericin sodium salt (Cat#S6653), cycloheximide (Cat#S7418) and Z-VAD-FMK (Cat#S7023) were purchased from Selleckchem (Houston, TX, USA). NP-40 lysis buffer (Cat#K1127) was purchased from Apexbio (Houston, TX, USA). NH₄Cl (Cat#A100621) was purchased from Sangon Biotech (Shanghai, China). β-Mercaptoethanol was from Thermo/Fisher (Cat#BP176100, Waltham, MA, USA). Details of other materials and suppliers are provided in the specific sections.

Wang F., Zhang M., Yuan M., Xia Z., Yang F., Zhang S., Lin T., Luo L., Tang J, & Zhang Y. (2023). A novel sorbicillinoid compound as a potent anti-inflammation agent through inducing NLRP3 protein degradation. British journal of pharmacology, 180(15), 1930-1948.

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Comprehensive Protocol for Cell Stress Assays

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Chiang L.W., Grenier J.M., Ettwiller L., Jenkins L.P., Ficenec D., Martin J., Jin F., DiStefano P.S, & Wood A. (2001). An orchestrated gene expression component of neuronal programmed cell death revealed by cDNA array analysis. Proceedings of the National Academy of Sciences of the United States of America, 98(5), 2814-2819.

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Comprehensive Compound Acquisition Protocol

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Pevonedistat hydrochloride (HY-10484), Ixazomib citrate (HY-10452), Delanzomib (HY-10454), Bortezomib (HY-10227), Carfilzomib (HY-10455), Pyrrolidinedithiocarbamate ammonium (HY-18738), Sitagliptin (HY-13749), GW843682X (HY-11003), Clofarabine (HY-A0005), Nocodazole (HY-13520) were all purchased from MedChemExpress.

Dai J., Zheng S., Falco M.M., Bao J., Eriksson J., Pikkusaari S., Forstén S., Jiang J., Wang W., Gao L., Perez-Villatoro F., Dufva O., Saeed K., Wang Y., Amiryousefi A., Färkkilä A., Mustjoki S., Kauppi L., Tang J, & Vähärautio A. (2024). Tracing back primed resistance in cancer via sister cells. Nature Communications, 15, 1158.

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Immunoprecipitation and Western Blot Analysis

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HEK293T cells and shelduck embryo fibroblast (DEF) cells were maintained at 37°C in DMEM (Gibco), enriched with 10% FBS (Gibco). Anti-HA (26183), anti-MYC (MA1-980), anti-FLAG (MA1-91878) and anti-GAPDH (MA515738) antibodies were purchased from Invitrogen. Anti-HA agarose beads (26182) and anti-V5 agarose beads (A7345) were purchased from Invitrogen and Sigma, respectively. Low molecular weight (LMW)-poly(I:C) and anti-V5 (ab27671) antibody were obtained from Invivogen and Abcam, respectively. IRDye^® 800CW goat anti-rabbit IgG (C50331-03) and IRDye^® 800CW goat anti-mouse IgG (C41028-02) secondary antibodies were from LI-COR. DMSO, MG132, bortezomib, bafilomycin A1 (Baf A1), chloroquine (CQ) and 3-Methyladenine (3-MA) were purchased from MedChemExpress.

Li W., Song Y., Du Y., Huang Z., Zhang M., Chen Z., He Z., Ding Y., Zhang J., Zhao L., Sun H, & Jiao P. (2023). Duck TRIM29 negatively regulates type I IFN production by targeting MAVS. Frontiers in Immunology, 13, 1016214.

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Comprehensive Cell Culture Protocol

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All chemicals and reagents were obtained from commercial suppliers. DAPI (#D9542) was purchased from Sigma (St. Louis, MO, USA). MG132 (#HY-13259), bortezomib (HY-10227) and cisplatin (#HY-17394) were purchased from MedChemExpress (Junction, NJ, USA). (S)-(+)-Camptothecin (#C111282) was obtained from Aladdin Chemical Co. (Shanghai, China). Shikonin (#517-89-5), Z-VAD-FMK (#S7023), etoposide (#S1225) and gemcitabine (#S1149) were from Selleckchem (Huston, TX, USA). Cells were grown in DMEM (U2OS, PANC-1, SW620 and MDA-MB-231) or RPMI-1640 (A549) supplemented with 10% fetal bovine serum (ExCell bio, China) and 1% penicillin–streptomycin (Gibco) at 37 °C in 5% CO₂ and 98% humidity incubator. Lipofectamine RNAiMAX (#13778030) was purchased from Thermo/Fisher Scientific (Carlsbad, CA, USA). Matrigel was purchased from Corning (Glendale, AR, USA).

Wang F., Jin S., Mayca Pozo F., Tian D., Tang X., Dai Y., Yao X., Tang J, & Zhang Y. (2021). Chemical screen identifies shikonin as a broad DNA damage response inhibitor that enhances chemotherapy through inhibiting ATM and ATR. Acta Pharmaceutica Sinica. B, 12(3), 1339-1350.

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Cell Lines, Small Molecules, and Formulations

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The following cell lines were purchased from the American Type Culture Collection (ATCC): K-562R, THP-1, KASUMI-6, AML-193, SUP-B15, KU-812, U-937, CA46, SU-DHL-6, REH, NCI-H929, JVM-2, JEKO-1, MAVER-1, MINO, REC-1, Z-138, RPMI8226, SU-DHL-4, and SU-DHL-10. OCI-LY1 was purchased from the German Collection of Microorganisms and Cell Cultures GmbH (DSMZ). DOHH-2, OCI-LY2, OCI-LY-8, TMD8, HAP1, AA8, UV5, UV20, UV21, and EM9 were available at and authenticated by our contract research organizations. Human fibroblast cells GM00637 and GM05849 were purchased from Coriell Institute for Medical Research. All the cell lines were cultured in medium according to the vendors’ recommendation in cell culture incubators set at 37°C with 5% CO2. LP-284 was provided by Lantern Pharma Inc., and stored at −80°C. LP-284 was formulated from powder by dissolving in 5% EtOH and 95% saline (in vivo studies) or DMSO (in vitro studies). Bortezomib was purchased from MedChemExpress (Cat. HY-10227) and formulated with 1% DMSO and 99% Saline. Ibrutinib was purchased from MedChemExpress (Cat. HY-10997) and formulated with 10% DMSO, 40% PEG 300, 5% Tween 80, and 45% saline. Spironolactone was purchased from Selleckchem (Catalog No.S4054) and formulated with 100% DMSO.

Zhou J., Sturtevant D., Love C., Kulkarni A., Biyani N., Kathad U., Thacker E., Dave S, & Bhatia K. (2023). LP-284, a small molecule acylfulvene, exerts potent antitumor activity in preclinical non-Hodgkin's lymphoma models and in cells deficient in DNA damage repair. Oncotarget, 14, 597-611.

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Angiogenic Inhibitors Modulate DIPG Tumor Growth

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Alisertib (MedChemExpress, Monmouth Junction, NJ), bortezomib (MedChemExpress), panobinostat (MedChemExpress), ponatinib (MedChemExpress), MSN1-Leu (synthesized in our lab, [20 (link)]) were sterilely prepared and dissolved in 0.5% DMSO and sterile PBS. Bevacizumab (SelleckChem) is water soluble, and was directly dissolved in sterile PBS. Drugs were administered by pipetting 15μL of drug solution directly on top of (but not contacting) the CAM tumor on embryonic development day 11, 13, 15. Each experiment contained 1–5 biological replicates per treatment group (at time of ultrasound) and there were at least three experimental replicates for each drug (n = 3–24 per drug treatment). Vehicle treatments (0.5% DMSO in sterile PBS) from all drug experimental replicates were combined to form one large vehicle group for further analysis (DIPGXIIIp* n = 38, DIPGIV n = 19).

Power E.A., Fernandez-Torres J., Zhang L., Yaun R., Lucien F, & Daniels D.J. (2022). Chorioallantoic membrane (CAM) assay to study treatment effects in diffuse intrinsic pontine glioma. PLoS ONE, 17(2), e0263822.

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Diverse Compounds for Biological Research

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Paclitaxel (S1150), tyrphostin AG 1296 (S8024), anagrelide (S3172),
levonorgestrel (S1727), Deoxycorticosterone acetate (S4243), drospirenone
(S1377), and norethindrone (S4040) were purchased from Selleck Chemicals.
Dofequidar (SML0938–5MG),
N,N′-Bis(salicylidene)-o-phenylenediamine vanadium(IV) oxide complex
(68541), and vanadium(IV) oxide sulfate hydrate (233706) were purchased from
Sigma-Aldrich. Tepoxalin (T103205) was purchased from Toronto Research Chemicals
and WuXi AppTec (custom synthesis). DCEBIO (1422) and Zardaverine (1046) were
purchased from Tocris. Disulfiram (HY-B0240), Bortezomib (HY-10227), and
Tanaproget (HY-15606) were purchased from MedChemExpress. BMOV (FB18735) was
purchased from Carbosynth. Tetrathiomolybdate (AC389530010) was purchased from
Thermo Fisher Scientific. Danazol (1500220) was purchased from Microsource.
Gestrinone (Prestw-1267) was purchased from Prestwick.

Corsello S.M., Nagari R.T., Spangler R.D., Rossen J., Kocak M., Bryan J.G., Humeidi R., Peck D., Wu X., Tang A.A., Wang V.M., Bender S.A., Lemire E., Narayan R., Montgomery P., Ben-David U., Garvie C.W., Chen Y., Rees M.G., Lyons N.J., McFarland J.M., Wong B.T., Wang L., Dumont N., O’Hearn P.J., Stefan E., Doench J.G., Harrington C.N., Greulich H., Meyerson M., Vazquez F., Subramanian A., Roth J.A., Bittker J.A., Boehm J.S., Mader C.C., Tsherniak A, & Golub T.R. (2020). Discovering the anti-cancer potential of non-oncology drugs by systematic viability profiling. Nature cancer, 1(2), 235-248.

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