Analysis of replicating DNA were performed according to the methods described previously32 (link). In brief, cells were pulse-labeled with 50 μM CldU for 15 min, followed by second labeling with 50 μM IdU for 15 min before analysis. Cells were harvested and DNA fibers were stretched onto glass slides in a DNA lysis buffer (200 mM Tris-HCl at pH 7.4, 0.5% SDS, 50 mM EDTA). Single replicating DNA was detected with rat anti-CldU (Novus), mouse anti-IdU (Becton Dickinson) and anti-single-strand DNA (EMD Millipore) antibodies. Images were captured using the Nikon Eclipse TiE microscope and processed using NIS Elements AR imaging software.
Anti single strand dna
Manufactured by Merck Group
Anti-single-strand DNA is a lab equipment product designed to detect and analyze single-stranded DNA molecules. It functions by selectively binding to and stabilizing single-stranded DNA, enabling researchers to study DNA structure, repair, and other related processes.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anti single strand dna
1
Replicating DNA Analysis Protocol
2
Replicating DNA Analysis Protocol
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