Pen strep glut

Human lung adenocarcinoma A549 cells were cultured in Ham-F12 (Gibco) supplemented with 10% fetal bovine serum (FBS, Sigma-Aldrich) and 100 U/mL penicillin-streptomycin-glutamine (Pen-Strep-Glut, Lonza) mixture. MDCK cells were cultured in Eagle’s minimal essential medium (EMEM; Lonza) plus 10% FBS, 1% Hepes 1 M, 1% sodium bicarbonate (Lonza), 1× non-essential amino acid solution, and 100 U/mL Pen-Strep-Glut mixture. A subclone 118 of Vero-WHO cells (Vero-118 (36 (link)) was cultured in Iscove’s modified Dulbecco’s medium (Lonza) supplemented with 10% FBS, and 100 U/mL Pen-Strep-Glut mixture. Jurkat cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 Medium (Gibco), supplemented with 10% FBS, 100 U/mL Pen-Strep, and 1% Hepes 1 M (all Gibco). All cells were cultured at 37°C and 5% CO₂. Monocytic THP-1 cells were cultured in RPMI 1640 (Gibco), supplemented with 10% FBS, 0.05 mM 2-mercaptoethanol (Gibco), and 100 U/mL Pen-Strep (Sigma-Aldrich).