The 1H-NMR (600 MHz) and 13C-NMR (150 MHz) spectra were measured in CDCl3 or DMSO-d6 using a JNM-ECZ600R spectrometer (JEOL, Tokyo, Japan) at room temperature, and the chemical shifts given as a δ (ppm) scale with tetramethylsilane (TMS) as the internal standard. The FAB-MS was measured using a JEOL JMS-HX110 mass spectrometer and acquired in a glycerol matrix. HPLC was conducted using a Waters machine equipped with a 1525 binary pump and a 2489 UV/Vis detector (Waters, Massachusetts, USA). Separation was carried out using a Cosmosil 5C18 MS-II column (20.0 mm × 250 mm, ODS, 5 µm; Nacalai Tesque, Kyoto, Japan). Apoptosis analyses were carried out using a Muse Cell Analyzer (Merck KGaA, Darmstadt, Germany).
Jms hx110 mass spectrometer
Manufactured by JEOL
Sourced in Germany
The JMS-HX110 is a high-performance mass spectrometer manufactured by JEOL. It is designed to provide accurate and reliable mass analysis for a wide range of applications. The JMS-HX110 utilizes a high-resolution double-focusing mass analyzer to deliver precise mass measurements and high-quality data.
Automatically generated - may contain errors
Lab products found in correlation
1525 binary pump, by Waters Corporation (1 mentions)
2489 uv vis detector, by Waters Corporation (1 mentions)
Cosmosil 5c18 ms 2 column, by Nacalai Tesque (1 mentions)
Muse cell analyzer, by Merck Group (1 mentions)
Jnm ecz600r spectrometer, by JEOL (1 mentions)
Dip 360 polarimeter, by Jasco (1 mentions)
60f254 silica gel, by Merck Group (1 mentions)
Ac 300 500 spectrometers, by Bruker (1 mentions)
Qstarxl instrument, by Thermo Fisher Scientific (1 mentions)
Uv 3200, by Hitachi (1 mentions)
302 a spectrophotometer, by Jasco (1 mentions)
4 protocols using jms hx110 mass spectrometer
1
NMR, MS, and Apoptosis Analysis Protocol
2
Analytical Characterization of Compound 1
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Optical rotation for compound 1 was measured on a DIP 360 polarimeter (JASCO, Tokyo, Japan). IR spectrum was recorded on a spectrophotometer with ATR-Tensor 37 by Bruker. JMS HX 110 Mass spectrometer (JEOL, Freising, Germany) was used to acquire the mass spectra. 1H and 13C NMR spectra were recorded on AM 600 NMR spectrometer (Bruker, Fallanden, Switzerland), and the chemical shift values were recorded in ppm (δ) units, whereas the coupling constants (J) were reported in Hz. The Final purification of compound 1 was carried out by using recycling HPLC (JAI), and chloroform was used as the mobile phase in a series of two silica columns; JAIGEL-1H and JAIGEL-2H (Serial No. J60-3E13, JAI, Yokohama, Japan). The TLC experiments were carried out on aluminum sheets pre-coated with 60F-254 silica gel (E. Merck, Darmstadt, Germany), which were developed in a mobile phase of 1% MeOH/dichloromethane to 5% MeOH/dichloromethane. The developed TLC plates were then sprayed in ceric sulphate and heated to observe the UV-inactive compounds.
3
Comprehensive Analytical Characterization Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Melting points were assigned in
open capillaries by a melting-point
equipment (SMP10). Thin-layer chromatography (TLC) plates precoated
with 0.25 mm thick layers of silica gel 60 F254 were used to monitor
the reactions as they unfolded (Merck). UV light illumination and/or
treatment with 10% H2SO4 in an aqueous methanol
solution were used for detection. The process of flash chromatography
utilized commercial alumina. Infrared (IR) spectra were recorded using
a Bruker Alpha-I ATR FTIR spectrophotometer and are expressed in v (cm–1). The 1H NMR, 13C NMR, and 2D NMR spectra were acquired using Bruker AC 300–500
spectrometers in CDCl3 and DMSO-d6 with tetramethylsilane as the internal standard. Coupling
constants are described in Hz, and chemical changes are described
in ppm. The incorporation of D2O verified the OH and NH
assignments. Both the Jeol JMS.600H and Finnigan MAT312 mass spectrometers
were used to capture the EI mass spectra.The Finnigan MAT 95XP was
used to record the mass spectra of HREI experiments. A Jeol JMS HX110
mass spectrometer was used to capture the FAB-MS data. ESI-MS spectra
were determined with an Applied Biosystems QStarXL instrument. Using
Flash EA-1112 equipment, CHNS microanalysis was carried out.
open capillaries by a melting-point
equipment (SMP10). Thin-layer chromatography (TLC) plates precoated
with 0.25 mm thick layers of silica gel 60 F254 were used to monitor
the reactions as they unfolded (Merck). UV light illumination and/or
treatment with 10% H2SO4 in an aqueous methanol
solution were used for detection. The process of flash chromatography
utilized commercial alumina. Infrared (IR) spectra were recorded using
a Bruker Alpha-I ATR FTIR spectrophotometer and are expressed in v (cm–1). The 1H NMR, 13C NMR, and 2D NMR spectra were acquired using Bruker AC 300–500
spectrometers in CDCl3 and DMSO-d6 with tetramethylsilane as the internal standard. Coupling
constants are described in Hz, and chemical changes are described
in ppm. The incorporation of D2O verified the OH and NH
assignments. Both the Jeol JMS.600H and Finnigan MAT312 mass spectrometers
were used to capture the EI mass spectra.The Finnigan MAT 95XP was
used to record the mass spectra of HREI experiments. A Jeol JMS HX110
mass spectrometer was used to capture the FAB-MS data. ESI-MS spectra
were determined with an Applied Biosystems QStarXL instrument. Using
Flash EA-1112 equipment, CHNS microanalysis was carried out.
4
Spectroscopic Analysis of Chemical Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The spectra of UV were reported on spectrophotometric instrument Hitachi UV-3200. IR spectral data were measured using a JASCO 302-A spectrophotometer. EI–MS (m/z) were recorded on JEOL JMS-HX-110 mass spectrometer. At 400 MHz, the spectra of 1H-NMR were observed on the instrument named Bruker AM-400 AMX spectrometers with the respective frequency data system. TMS of compounds was used as a reference (mainly internally). The spectra of 13C-NMR (broadband and DEPT or GASPE) were accomplished on the same instruments at 75 MHz, 100 MHz, and 125 MHz, respectively. The values of the chemical shift were expressed as ppm (δ) and the coupling constants (J) values were expressed in Hz.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!