Cd45 cd3 cd4 cd8 four color antibody

Manufactured by BD

The CD45+/CD3+/CD4+/CD8+ four-color antibody is a lab equipment product designed for cellular analysis. It enables the simultaneous identification and enumeration of CD45+, CD3+, CD4+, and CD8+ cell populations in a single sample.

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Lab products found in correlation

Trucount tube, by BD (2 mentions) Real time pcr m2000 system, by Abbott (1 mentions)

Spelling variants of this product

CD8 antibody (7 citations) CD3/CD8/CD45/CD4 (2 citations)

2 protocols using cd45 cd3 cd4 cd8 four color antibody

Quantifying CD4+ T-cells and Viral Load

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CD4⁺ T-cell absolute count (cells/μl) were determined in fresh whole blood samples from AHI individuals using Trucount Tubes and CD45⁺/CD3⁺/CD4⁺/CD8⁺ four-color antibody (BD Biosciences) by flow cytometry. Plasma HIV viral load (copies/ml) was quantified by automated Real-Time PCR M2000 system (Abbott Molecular Inc., Des Plaines, IL, USA), with a detection limit of 40 copies/ml.

Song A., Li Z., Luo Z., Lu X., Wang R., Liu L., Xia W., Wan Z., Zhang T., Su B., Jiang W, & Wu H. (2020). Effects of Early and Delayed Antiretroviral Therapy on Plasma Anti-CD4 Autoreactive IgG and Its Association With CD4+ T-Cell Recovery in Acute HIV-Infected Individuals. Frontiers in Pharmacology, 11, 449.

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Quantifying Plasma HIV-1 Viral Load

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Plasma HIV-1 viral load (pVL) was quantified using automated RealTime PCR-based m2000 system (Abbott Molecular Inc., Des Plaines, IL, USA), with a detection limit of 40 copies/ml. CD4⁺ T cell absolute count were determined in fresh whole blood by using Trucount Tubes and CD45/CD3/CD4/CD8 four-color antibody (BD Biosciences) according to the manufacturer’s protocol.

Xia H., Jiang W., Zhang X., Qin L., Su B., Li Z., Sun J., Zhang Y., Zhang T., Lu X, & Wu H. (2018). Elevated Level of CD4+ T Cell Immune Activation in Acutely HIV-1-Infected Stage Associates With Increased IL-2 Production and Cycling Expression, and Subsequent CD4+ T Cell Preservation. Frontiers in Immunology, 9, 616.

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