Anti cd11c bv605

Manufactured by BioLegend

Anti-CD11c-Bv605 is a fluorescence-labeled antibody used for the detection and identification of cells expressing the CD11c antigen. CD11c is a cell surface marker found on certain types of dendritic cells and macrophages. This antibody is conjugated to the Brilliant Violet 605 fluorochrome, which can be detected using flow cytometry.

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Anti-CD11c (97 citations) CD11c-BV605 (12 citations) BV605 anti-mouse Cd11c (2 citations)

2 protocols using anti cd11c bv605

Immune Cell Identification in Mouse BALF and Lung

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For the identification of immune cell populations, mouse BALF and lung cells were stained in the presence of counting beads (15.45 μm DragonGreen, Bangs Laboratories Inc., FS07F) with LIVE/DEAD stain (Invitrogen, L23105A) and an antibody mixture of anti-CD45-AF700 (BioLegend, 103127), anti-CD11b-AF594 (BioLegend, 101254), anti-CD11c-Bv605 (BioLegend, 117334), anti-SiglecF-AF647 (BD, 562680), anti-MHCII-APC Cy7 (BioLegend, 107628), anti-Ly6C-Bv421 (BioLegend, 128032), and anti-Ly6G-PerCP-Cy5.5 (BioLegend #127616), each at a concentration of 1:200 in PBS, for 1 h at 4°C. After washing, the cells were stored in 2% paraformaldehyde (PFA, Electron Microscopy Sciences, 15714-S) until analysis on the BD LSRII (BD Biosciences) using FACSDiva v9. Flow cytometry was analyzed with FlowJo v10. Mouse BAL and lung cells were identified as follows:
alveolar macrophages: CD45⁺CD11b^+/−SiglecF⁺CD11c⁺;
monocytes: CD45⁺CD11b⁺SiglecF⁻MHCII⁻CD11c⁻Ly6G⁻Ly6C^+/−;
neutrophils: CD45⁺CD11b⁺SiglecF⁻ MHCII⁻CD11c⁻Ly6G⁺Ly6C^+/−.

Wong Fok Lung T., Charytonowicz D., Beaumont K.G., Shah S.S., Sridhar S.H., Gorrie C.L., Mu A., Hofstaedter C.E., Varisco D., McConville T.H., Drikic M., Fowler B., Urso A., Shi W., Fucich D., Annavajhala M.K., Khan I.N., Oussenko I., Francoeur N., Smith M.L., Stockwell B.R., Lewis I.A., Hachani A., Baskota S.U., Uhlemann A.C., Ahn D., Ernst R.K., Howden B.P., Sebra R, & Prince A. (2022). Klebsiella pneumoniae induces host metabolic stress that promotes tolerance to pulmonary infection. Cell metabolism, 34(5), 761-774.e9.

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Multiparametric Flow Cytometry Immunophenotyping

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Blood samples were incubated with ACK lysis solution to remove erythrocytes. Single-cell suspension of splenic cells was obtained by gently pressing spleens through 70 μm cell strainers (Corning, cat. no. 352350). Bone marrow cells were harvested from one femur per mouse. Peritoneal lavage was performed by repeatedly flushing the peritoneal cavity with 10 ml PBS/2% FCS. Single-cell suspensions from the different tissues were resuspended in PBS/2% FCS and briefly incubated with Fc blocking antibodies (clone 2.4G2, purified in-house). Cells were then stained with the following fluorescently labelled monoclonal antibodies: anti-CD8α-FITC (ThermoFisher, cat. no. 11-0081-81), anti-TCRβ-PE (BioLegend, cat. no. 109208), anti-CD11b-PerCP-Cy5.5 (BioLegend, cat. no. 101228), anti-Gr-1-APC (ThermoFisher, cat. no. 17-5931-82), anti-CD19-PE-Cy7 (BioLegend, cat. no. 115520), anti-CD45-BV785 (BioLegend, cat. no. 103149), anti-CD4-BV711 (BioLegend, cat. no. 100447), anti-CD11c-BV605 (BioLegend, cat. no. 117333) and anti-Ly-6G-BV421 (BioLegend, cat. no. 127628). For nonviable cell exclusion, Fixable Viability Dye eFluor^® 780 (ThermoFisher, cat. no. 65-0865-18) was used. Stained cells were then fixed with 4% formalin and resuspended in PBS/2% FCS for flow cytometric analysis using a LSRFortessa (BD Biosciences). FACS data were then analysed with FlowJo software (RRID:SCR_008520, version 10.0.7).

Pereira J.A., Gerber J., Ghidinelli M., Gerber D., Tortola L., Ommer A., Bachofner S., Santarella F., Tinelli E., Lin S., Rüegg M.A., Kopf M., Toyka K.V, & Suter U. (2020). Mice carrying an analogous heterozygous dynamin 2 K562E mutation that causes neuropathy in humans develop predominant characteristics of a primary myopathy. Human Molecular Genetics, 29(8), 1253-1273.

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