Antibodies (Ab) against cytochrome c (cat. no. ab133504), Bcl-2 (cat. no. ab196495), cytochrome c oxidase IV (COX IV) (cat. no. ab202554) and GAPDH (cat. no. ab181602) were purchased from Abcam. Phosphorylated (p)-Bcl-2 (Ser70) rabbit monoclonal (m)Ab (cat. no. #2827), p-JNK (Thr183/Tyr185) rabbit mAb (cat. no. #4668) and JNK rabbit mAb (cat. no. #9258) were obtained from Cell Signaling Technology, Inc. LC3 rabbit mAb (cat. no. sc-398822) was purchased from Santa Cruz Biotechnology, Inc. The malondialdehyde (MDA) Assay kit (TBA method) (cat. no. A003-1) and H2O2 Assay kit (cat. no. A064-1) were obtained from Nanjing Jiancheng Bioengineering Institute. An In Situ Cell Death Detection kit was obtained from Roche Diagnostics GmbH. Dexamethasone (DXM) was purchased from Sigma-Aldrich (Merck KGaA). Tat-SabKIM1 (GFE SLS VPS PLD LSG PRV VAPPRRRQRRKKRG-NH2) was purchased from NeoPeptide. Seawater (osmolality 1,300 mmol/l; pH 8.2; 26.518 g/l NaCl; 3.305 g/l MgSO4; 2.447 g/l MgCl2; 1.141 g/l CaCl2; 0.725 g/l KCl; 0.202 g/l NaHCO3; 0.083 g/l NaBr) was prepared according to the major composition of the East China Sea provided by the Chinese Ocean Bureau (23 (link)).
Lc3 rabbit mab
Manufactured by Cell Signaling Technology
Sourced in United States
LC3 rabbit mAb is a monoclonal antibody that recognizes the microtubule-associated protein 1 light chain 3 (LC3) protein. LC3 is a key marker for autophagy, a cellular process involved in the degradation and recycling of cellular components.
Automatically generated - may contain errors
Lab products found in correlation
Dexamethasone dxm, by Merck Group (1 mentions)
In situ cell death detection kit, by Roche (1 mentions)
Beclin1 rabbit mab, by Cell Signaling Technology (1 mentions)
Mouse anti β actin mab, by Cell Signaling Technology (1 mentions)
Anti β actin mouse monoclonal antibody, by Cell Signaling Technology (1 mentions)
Anti rabbit igg hrp linked antibody, by Cell Signaling Technology (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Gemini Bio (1 mentions)
2 protocols using lc3 rabbit mab
1
Seawater-Induced Oxidative Stress Assays
2
Autophagy Pathway Regulation in Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The following reagents were used: EMDM (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA); FBS (Gemini Bio Products, West Sacramento, CA, USA); TRIzol (Ambion; Thermo Fisher Scientific, Inc.); MDC (Tiangen Biotech Co., Ltd., Beijing, China); WIP organization cell lysis solution (Boaoseng, Beijing, China); RNase Away (Ed lai, Beijing, China); BCA protein concentration determination kit (Blue skies, China); SDS-PA (Nanjing KeyGen Biotech. Co. Ltd., Nanjing, China); Reverse transcription kit (Lisu, Shanghai, China); Primers (Hongxun, Suzhou, China); High sensitive chemiluminescence detection kit (Qihaifutan, Shanghai, China); TRAP1 Rabbit mAb (Cell Signaling Technology, Inc., Danvers, MA, USA); Beclin1 Rabbit mAb (Cell Signaling Technology, Inc.); LC3 Rabbit mAb (Cell Signaling Technology, Inc.); Anti-rabbit IgG, HRP-linked Antibody (Isotype: Goat; Cell Signaling Technology, Inc.); Mouse anti β-actin mAb (Zhongshanjinqiao, China); Anti-β-actin Mouse Monoclonal Antibody (Isotype: Goat; Zhongshanjinqiao, China); and Protein Simple (San Jose, CA, USA).
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