Gonak hypromellose ophthalmic demulcent solution

Manufactured by Akorn

Sourced in United States

GONAK hypromellose ophthalmic demulcent solution is a sterile, preservative-free eye drop formulation. It contains hypromellose, a viscosity-increasing agent that provides lubrication and moisture to the eye.

Automatically generated - may contain errors

Lab products found in correlation

Tropicamide ophthalmic solution, by Bausch & Lomb (2 mentions) Tropicamide ophthalmic solution, by Akorn (2 mentions) Tetracaine hydrochloride ophthalmic solution, by Bausch & Lomb (1 mentions) Balanced salt solution, by Alcon (1 mentions) Phenylephrine hydrochloride ophthalmic solution, by Bioteck (1 mentions) Vitra 532 nm, by Lumibird Medical (1 mentions) Tropicamide, by Alcon (1 mentions) Proparacaine hydrochloride, by Alcon (1 mentions) Phenylephrine, by Alcon (1 mentions) Prism 9, by GraphPad (1 mentions) Celeris system, by Diagnosys (1 mentions) Micron 4 camera, by Phoenix Pharmaceuticals (1 mentions)

Spelling variants of this product

Gonak (18 citations) Hypromellose (6 citations) Hypromellose ophthalmic demulcent solution (5 citations) Gonak solution (3 citations)

8 protocols using gonak hypromellose ophthalmic demulcent solution

Anesthetic and Ophthalmic Solutions for Animal Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

All reagents were in sterile packages, and medication and drops were filtered to ensure sterility. They included the following:

•

Ketamine hydrochloride solution 100 mg/mL (Dechra Veterinary Products, Overland Park, KS)

•

Xylazine 20 mg/mL (AnaSed, Shenandoah, IA)

•

Phenylephrine hydrochloride ophthalmic solution 2.5% (Paragon BioTeck, Portland, OR)

•

Tropicamide ophthalmic solution 1% (Bausch & Lomb, Rochester, NY)

•

GONAK hypromellose ophthalmic demulcent solution 2.5% (Akorn Pharmaceuticals, Lake Forest, IL)

•

Tetracaine hydrochloride ophthalmic solution 0.5% (Bausch & Lomb)

•

Balanced salt solution 15 mL (Alcon, Fort Worth, TX)

•

Clidox-S solution (Pharmacal Research Laboratories, Waterbury, CT)

Movahedan A., Barba H., Spedale M., Deng N., Arvans D., Nadeem U., Leone V., Chang E.B., Theriault B, & Skondra D. (2021). Gnotobiotic Operations and Assembly for Development of Germ-Free Animal Model of Laser-Induced Choroidal Neovascularization. Translational Vision Science & Technology, 10(9), 14.

+ Open protocol

+ Expand

Comprehensive Electroretinography Evaluation

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

ERG tests were administered to both eyes of all mice using previously described methods.⁴ (link)^,³⁸ (link)^,⁵⁹ (link) ERG testing was performed 6-, 8-, and 10-week post-injection. Mice were dark-adapted for 12 h prior to anesthetization with 0.1 mL/10 g B.W. of 1 mL of 100 mg/mL ketamine and 0.1 mL of 20 mg/mL xylazine in 8.9 mL PBS, which was injected i.p., as previously described.⁴ (link) During anesthetization, mice were placed on heating pads to maintain their body temperature at 37°C. Mouse eyes were dilated with one drop per eye of Tropicamide Ophthalmic Solution (1%; Akorn). Electrodes were placed on the corneas, and Gonak Hypromellose Ophthalmic Demulcent Solution (2.5%; Akorn) was applied to the eyes to prevent corneal scarring.
ERG recordings were simultaneously for both eyes. As previously described, pulses of 0.00130 cd/m² and 3 cd/m² (White-6500K) were employed.⁴ (link)^,⁴³ (link)^,⁴⁴ (link)^,⁵⁶ (link)^,⁶⁰ (link) This allowed the specific testing of rod and cone functionality so that the individual and combined function could be analyzed.

Nolan N.D., Cui X., Robbings B.M., Demirkol A., Pandey K., Wu W.H., Hu H.F., Jenny L.A., Lin C.S., Hass D.T., Du J., Hurley J.B, & Tsang S.H. (2024). CRISPR editing of anti-anemia drug target rescues independent preclinical models of retinitis pigmentosa. Cell Reports Medicine, 5(4), 101459.

+ Open protocol

+ Expand

Retinal Vein Occlusion Induction via Dye-Enhanced Photothrombosis

Check if the same lab product or an alternative is used in the 5 most similar protocols

To create the retinal vein occlusion in the retinal veins, dye-enhanced photothrombosis was performed as defined previously by Oncel et al. and Nguyen et al.^{45 (link),46 (link)}. In brief, a contact lens (Volk H-R Wide Field, laser spot 2x magnification, Volk Optical Inc, Mentor, OH, USA) coupled with the cornea of the rabbit eye using 2.5% Gonak Hypromellose Ophthalmic Demulcent Solution (Akorn, Lake Forest, IL, USA). The target veins were observed and defined from the slit lamp. Then, Rose Bengal with concentration of 50 mg/mL was intravenously injected into the rabbit. 5–10 seconds after the injection, a 532-nm green laser light (power = 150–300 mW, spot size = 75 µm, and the irradiation time = 0.5 s per spot) was used to shined into the rabbit eye (Vitra 532 nm, Quantel Medical, Cournon d’Auvergne, France). Each the retinal vein received twenty shots of the laser illuminations (150 mW) at the same position until the blood vessel was completely occluded and the blood flow was stopped. In addition, 20 shots of laser light was further applied at the power of 300 mW to inhibit reopening of the vein³⁸.

Nguyen V.P., Li Y., Zhang W., Wang X, & Paulus Y.M. (2019). High-resolution multimodal photoacoustic microscopy and optical coherence tomography image-guided laser induced branch retinal vein occlusion in living rabbits. Scientific Reports, 9, 10560.

+ Open protocol

+ Expand

Assessing Retinal Function via Focal ERG

Check if the same lab product or an alternative is used in the 5 most similar protocols

Electroretinogram (ERG) recordings were performed using a focal ERG module attached to a retinal imaging microscope (model Micron IV, Phoenix Technology Group). Briefly, mice were dark adapted overnight and prepared for the experiment under dim red light. The mice were anesthetized with ketamine (100 mg/kg) and xylazine (10 mg/kg) and received topical proparacaine hydrochloride (0.5%; Alcon) via eye drops. Pupils were dilated with tropicamide (1%; Alcon) and phenylephrine (2.5%; Alcon) and were lightly coated with GONAK hypromellose ophthalmic demulcent solution (2.5%; Akorn). The lens of the Micron IV was placed directly on the cornea, and a reference electrode was placed on the mouse head. Scotopic responses were elicited with a series of flashes of increasing light intensities from −1.7 to 2.2 cd/s/m². Photopic responses were elicited under rod-desensitizing background light with a series of flashes of increasing light intensities from −0.5 to 2.8 cd/s/m². Values of a- and b-wave were extracted and plotted for comparisons between groups of interest.

Kisseleff E., Vigouroux R.J., Hottin C., Lourdel S., Thomas L., Shah P., Chédotal A., Perron M., Swaroop A, & Roger J.E. (2021). Glycogen Synthase Kinase 3 Regulates the Genesis of Displaced Retinal Ganglion Cells3. eNeuro, 8(5), ENEURO.0171-21.2021.

+ Open protocol

+ Expand

Retinal Imaging in Anesthetized Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were anesthetized by intraperitoneal injection of ketamine hydrochloride (86.9 mg/kg) and xylazine (10 mg/kg). Pupils were dilated using 1% tropicamide ophthalmic solution (Akorn, Inc.; Lake Forest, IL) and a drop of 2.5% Gonak™ hypromellose ophthalmic demulcent solution (Akorn, Inc.) was applied each eye to prevent drying and irritation. Eyes were imaged using a Micron III rodent fundus imaging microscope equipped with image-guided 830 nm OCT module (Phoenix Research Laboratories; Pleasanton, CA) with Micron OCT software Version 7.

Poria D., Sun C., Santeford A., Kielar M., Apte R.S., Kisselev O.G., Chen S, & Kefalov V.J. (2022). EML1 is essential for retinal photoreceptor migration and survival. Scientific Reports, 12, 2897.

+ Open protocol

+ Expand

Standardized Electroretinography in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were dark adapted overnight. Mice were anesthetized using a ketamine/xylazine mixture (87.5 mg/kg ketamine, 12.5 mg/kg xylazine), and 1% tropicamide ophthalmic solution was applied for 3 min prior to the ERG procedure. GONAK hypromellose ophthalmic demulcent solution (NDC 17478-064, Akorn) was applied on the eyes prior to electrode placement. ERG was performed using the Celeris system from Diagnosys (Lowell, MA). To assess rod function, mice were subjected to dim flashes of 0.01 cd s/m² under dark-adapted conditions. Combined rod-cone function was measured by bright flashes of 3.0 cd s/m² under dark-adapted conditions. To assess cone function, mice were light adapted for 10 min to bleach rods in the retina. Then, two different measures, one involving isolated 3.0 cd s/m² bright flashes and the other using a train of 5-Hz flickering light, were used to evaluate the functions of cones. These tests are standardized ERG testing protocols established by the ISCEV.⁴² (link) Statistical analysis of ERG amplitudes was performed with GraphPad PRISM 9.0 using two-way ANOVA. Values shown are averages ± standard error of the mean.

Hsu Y., Bhattarai S., Thompson J.M., Mahoney A., Thomas J., Mayer S.K., Datta P., Garrison J., Searby C.C., Vandenberghe L.H., Seo S., Sheffield V.C, & Drack A.V. (2022). Subretinal gene therapy delays vision loss in a Bardet-Biedl Syndrome type 10 mouse model. Molecular Therapy. Nucleic Acids, 31, 164-181.

+ Open protocol

+ Expand

Ophthalmic Imaging of Rodent Fundus

Check if the same lab product or an alternative is used in the 5 most similar protocols

Animals were anesthetized by intraperitoneal injection of a mixture of ketamine (50–75 mg/kg) and dexmedetomidine (1–5 mg/kg). The pupil was dilated with Tropicamide Ophthalmic Solution (Bausch & Lomb, Rochester, NY, USA), and covered with Gonak^™ hypromellose ophthalmic demulcent solution (Akorn, Lake Forest, IL, USA). Fundus photographs were taken with a Micron-IV camera (Phoenix Research Laboratories, Pleasanton, CA) with cornea-contacting lens (29 (link)). Spectral-domain optical coherence tomography (OCT) scan was performed on a Bioptigen Envisu Imaging System (Leica Microsystems Inc, Buffalo Grove, IL, USA) with a mouse retina lens.

Go Y.M., Zhang J., Fernandes J., Litwin C., Chen R., Wensel T.G., Jones D.P., Cai J, & Chen Y. (2020). MTOR-initiated Metabolic Switch and Degeneration in the Retinal Pigment Epithelium. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(9), 12502-12520.

+ Open protocol

+ Expand

In Vivo Fluorescence Fundoscopy in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fluorescence fundoscopy was performed using the Phoenix MICRON III (Bend, Oregon) small animal imaging system. Mice were anesthetized using a ketamine/xylazine mixture (87.5 mg/kg ketamine, 12.5 mg/kg xylazine), and 1% tropicamide ophthalmic solution was applied for 3 min prior to the procedure. GONAK hypromellose ophthalmic demulcent solution (NDC 17478-064, Akorn) was applied on the eye prior to imaging.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!