Zr 75 1

The human breast cancer cell lines MCF-10 A, MCF-7, MDA-MB-231, MDA-MB-453, BT-474, and SK-BR-3 were obtained from the Shanghai Cell Bank (Shanghai, China), and ZR75-1 and MDA-MB-468 were purchased from Procell Life Science and Technology (Wuhan, China). All cell lines were authenticated using short tandem repeat DNA profiling with no more than 10 passages and no mycoplasma contamination. Cells were cultured in RPMI 1640 medium (Invitrogen, Carlsbad, CA, USA) containing 10% fetal calf serum (Invitrogen), 100 IU/mL penicillin, and 100 µg/mL streptomycin (Sigma-Aldrich, St. Louis, MO, USA). The cells were cultured in sterile culture dishes at 37 °C in a 5% CO₂ atmosphere.

MCF7 (RRID: CVCL 0031) and T47D (RRID: CVCL 0553) cell lines were obtained from Guangzhou Cellcook Biotech Co. Ltd. (Guangzhou, China). HCC1954 (RRID: CVCL 1259) was purchased from Cobioer (Nanjing, China). MDA-MB-231 (RRID: CVCL 0062), MDA-MB-468 (RRID: CVCL 0419), SKBR-3 (RRID: CVCL 0033), MCF10A (RRID: CVCL 0598), and ZR-75-1 (RRID: CVCL 0588) were acquired from Procell Life Science & Technology Co., Ltd. (Wuhan, China). All specimens were cultured in standardized media and conditions. All cell lines were subjected to STR authentication. All experiments were performed using mycoplasma-free cells.