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Cd11c magnetic bead isolation

Manufactured by Miltenyi Biotec
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The CD11c magnetic bead isolation product is a laboratory tool used to isolate and extract CD11c-positive cells from biological samples. It functions by utilizing magnetic beads coated with antibodies specific to the CD11c surface marker, allowing for the targeted separation and collection of the desired cell population.

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Lab products found in correlation

Znso4, by Merck Group (2 mentions) Gentamicin, by MP Biomedicals (1 mentions) Gm csf, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Cytiva (1 mentions) Roswell park memorial institute (rpmi), by Lonza (1 mentions) Fcs express 4, by De Novo Software (1 mentions)

Spelling variants of this product

Magnetic beads (547 citations) CD11c magnetic beads (30 citations) Magnetic bead isolation (17 citations) CD11c+ beads (16 citations)

2 protocols using cd11c magnetic bead isolation

1

Histoplasma Infection of Dendritic Cells

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Bone marrow from femurs and tibias of ~8–12 week old mice were cultured in RPMI (BioWhittaker, MD) with 10% fetal bovine serum (HyClone Laboratories, Utah), 0.005% 2-mercaptoethanol,10 mg/L gentamicin (MP Biomedicals, LLC) and 10 ng/ml GM-CSF (Peprotech) for 6 days at 37°C and 5% CO2. DCs were isolated from floating cells using CD11c magnetic bead isolation (Miltenyi Biotec) and treated with 100 μM ZnSO4 (Sigma-Aldrich) and infected with H. capsulatum (217B) at a multiplicity of infection (MOI) of 0.5 yeasts per DC for 24 h at 37°C. Cells were harvested after 24 h for gene expression analysis or flow cytometry.
George M.M., Vignesh K.S., Landero Figueroa J.A., Caruso J.A, & Deepe GS J.r. (2016). Zinc Induces Dendritic Cell Tolerogenic Phenotype and Skews Regulatory T cell – Th17 Balance. Journal of immunology (Baltimore, Md. : 1950), 197(5), 1864-1876.
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2

Zinc-Induced Dendritic Cell Apoptosis

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DCs were isolated from floating cells using CD11c magnetic bead isolation (Miltenyi Biotec) and treated with 100 μM ZnSO4 (Sigma-Aldrich) and infected with an MOI of 0.5 yeasts for 24 h at 37°C. Cells were washed with 0.5% bovine serum albumin in PBS (pH 7.4) and stained with 5 μl 7AAD stain/106 cells for 5 mins at 4°C and analyzed by flow cytometry (Accuri C6). Data were analyzed using FCS Express 4, De Novo Software (CA).
George M.M., Vignesh K.S., Landero Figueroa J.A., Caruso J.A, & Deepe GS J.r. (2016). Zinc Induces Dendritic Cell Tolerogenic Phenotype and Skews Regulatory T cell – Th17 Balance. Journal of immunology (Baltimore, Md. : 1950), 197(5), 1864-1876.
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