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2 protocols using a 77636

1

Operant Oral Self-Administration of Ethyl Alcohol

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Ethyl alcohol (190 proof; McCormick Distilling Co., Weston, MO) was diluted to 15% with distilled water for operant oral self-administration sessions. SCH 23390, a D1 receptor antagonist, (Sigma, St. Louis, MO) and A-77636, D1 receptor agonist, (Tocris Bioscience, Ellisville, MO) were dissolved in artificial cerebral spinal fluid (aCSF), and the pH adjusted to 6.5 ± 0.1 for microinjections. The aCSF consisted of: 120.0 mM NaCl, 4.8 mM KCl, 1.2 mM KH2PO4, 1.2 mM Mg SO4, 25.0 mM NaHCO3, 2.5 mM CaCl2, and 10.0 mM D-glucose.
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2

Pharmacological Compounds for Cell Experiments

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Y-27632, forskolin, SB431542, formoterol, fenoterol, salbutamol, salmeterol, and fenoldopam were purchased from Cayman Chemical (Ann Arbor, MI). Dihydrexidine, A77636, A68930, and fenoldopam were purchased from Tocris Bioscience (Minneapolis, MN). Recombinant human TGF-β1 was purchased from PeproTech (Rocky Hill, NJ). Bovine serum albumin (BSA), 10% formalin and triton X-100 were purchased from Sigma-Aldrich (St. Louis, MO). Phosphate buffered saline (PBS) was purchased from Life Technologies (Carlsbad, CA).
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