A 1210477

The following drugs were dissolved in DMSO and stored at −20°C: WEHI-539 [32 (link)] (Apexbio); taxol (Sigma); nocodazole (Sigma); AZ138 [8 (link)] (AstraZeneca)); AZ3146 [43 (link)] (AstraZeneca); BI 2536 [38 (link)] (Boehringer Ingelheim); GSK923295 [39 (link),70 ]; MLN8054 [41 (link)] (Millennium Pharmaceuticals); ZM447439 [42 (link)] (Tocris); A-1210477 [31 (link)] (Medchemexpress). Tetracycline (Sigma) was dissolved in water, stored at −20°C, and used at concentrations indicated in the figure legends. Thymidine (Sigma) was dissolved in PBS at a concentration of 200 mM, and stored short-term at 4°C.

CADPE (>98%) was synthesized by the authors^{31 (link)} and dissolved in DMSO for in vitro assay or in hydroxypropyl-β-cyclodextrin for in vivo experiments. Inhibitors ABT737 (737 for Bcl-xl), A-1210477 (477 for Mcl-1), YM155 (155 for survivin), Bay 11-7085 (Bay for NF-κB), ruxolitinib (Rux for STAT3), 10058-F4 (F4 for c-Myc), and 4EGI-1 (4EGI for Cap-translation) and positive control drug regorafenib (Rego) were purchased from the MedChemexpress Co., Ltd. All CRC cells were obtained from the China Type Culture Collection (Shanghai) and normal colon fibroblast CCD-18Co cells from the Shanghai Bogoo Biotechnology Co., Ltd. HCT-8, HCT-15, and CT26.WT cells were cultured in RPMI-1640 (Gibco), HCT-116 and HT-29 cells in McCOY′5A (Gibco), SW620 cells in Leiboviz′s L15 (Gibco), and CCD-18Co cells in DMEM (Gibco), supplemented with 2 mM l-glutamine. All cells were grown in medium with 10% fetal bovine serum (FBS), penicillin (20 U/mL), and streptomycin (20 μg/mL). Cells were authenticated by STR profiling and routinely screened for the presence of Mycoplasma by EZ-PCR Mycoplasma test Kit (Biological Industries).

Without specific indication, the reagents obtained from Sigma-Aldrich Inc. (St. Louis, MO, USA) were used in this study, and cell culture supplements were the products of GIBCO/Life Technologies Inc. (Carlsbad, CA, USA). ABT-263 was obtained from Apexbio Technology LLC (Houston, TX, USA), and A-1210477 was the product of MedChem Express (Monmouth Junction, NJ, USA). MitoSOX Red, tetramethylrhodamine (TMRM), annexin V-FITC/propidium iodide (PI) apoptosis detection kit, and dichlorodihydrofluorescein diacetate (H₂DCFDA) were obtained from Molecular Probes (Eugene, OR, USA); and Z-DEVD-FMK was from Calbiochem (San Diego, CA, USA).