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Slc5a8

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SLC5A8 is a sodium-coupled monocarboxylate transporter that facilitates the uptake of short-chain fatty acids and lactate into cells. It plays a role in cellular energy metabolism and pH regulation.

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Lab products found in correlation

Claudin 5, by Abcam (2 mentions) α tubulin, by Cell Signaling Technology (2 mentions) Acetyl histone h4 lys5 8 12 16, by Merck Group (2 mentions) 3hh 4c10, by Abcam (2 mentions) Gpr43, by Abcam (2 mentions) Pierce bca protein assay, by Thermo Fisher Scientific (2 mentions) Semi dry transfer cell, by Bio-Rad (2 mentions) Occludin, by Abcam (2 mentions) Glyceryl tributyrate, by Merck Group (1 mentions) Tumor necrosis factor alpha tnf α, by R&D Systems (1 mentions) Pair fed control diet, by Dyets (1 mentions) Gpr109a, by Bioss Antibodies (1 mentions)

3 protocols using slc5a8

1

Glyceryl Tributyrate Dietary Modulation

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Glyceryl Tributyrate was purchased from Sigma-Aldrich (St. Louis, MO). Pair-fed control diet and modified Lieber-DeCarli high-fat diet were purchased from Dyets, Inc. (product number 710260; Bethlehem, PA). All primers for quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) were synthesized by Integrated DNA Technologies (Coralville, IA). Primary antibodies were purchased from the following companies: Occludin (Hycult Biotech, Plymouth Meeting, PA); GPR109A (Bioss, Woburn, MA); tumor necrosis factor-alpha (TNFα; R&D Systems, Minneapolis, MN); Zonula Occluden-1 (ZO-1) and SLC5A8 (Abcam, Cambridge, MA).
Cresci G.A., Bush K, & Nagy L.E. (2014). Tributyrin Supplementation Protects Mice from Acute Ethanol-Induced Gut Injury. Alcoholism, clinical and experimental research, 38(6), 1489-1501.
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2

Intestinal Epithelial Cell Protein Analysis

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CD326+ purified IECs were harvested from animals that received syngeneic (BALB/c → BALB/c) or allogeneic (C57BL/6J → BALB/c) BMT. Whole cell lysates were next obtained and protein concentrations determined with Pierce BCA Protein Assay (Thermo Scientific). Equal amounts of protein (20 µg) were separated by SDS-PAGE gel electrophoresis (120V, 1.5h) and subsequently transferred to polyvinylidene difluoride (PVDF) membrane using a Bio-Rad semi-dry transfer cell (Hercules, CA) (20V, 1h). The following antibodies were used to analyze the membranes with dilutions in accordance with the manufacturers specification sheet: α-tubulin (Cell Signaling, clone 11H10), Acetyl histone H4 (Lys5/8/12/16) (EMD Millipore, clone 3HH-4C10), SLC5A8 (abcam), GPR43 (abcam), Occludin (abcam, clone EPR8208), JAM (abcam, clone EP1042Y), and Claudin 5 (abcam). Secondary anti-rabbit antibody conjugated to HRP (Jackson ImmunoResearch, Cat No. 111-035-003) was used to detect primary antibodies, where needed. Densitometric analysis was performed using ImageJ software (National Institutes of Health; Bethesda, MA).
Mathewson N.D., Jenq R., Mathew A.V., Koenigsknecht M., Hanash A., Toubai T., Oravecz-Wilson K., Wu S.R., Sun Y., Rossi C., Fujiwara H., Byun J., Shono Y., Lindemans C., Calafiore M., Schmidt T.C., Honda K., Young V.B., Pennathur S., van den Brink M, & Reddy P. (2016). Gut microbiome derived metabolites modulate intestinal epithelial cell damage and mitigate Graft-versus-Host Disease. Nature immunology, 17(5), 505-513.
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3

Intestinal Epithelial Cell Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
CD326+ purified IECs were harvested from animals that received syngeneic (BALB/c → BALB/c) or allogeneic (C57BL/6J → BALB/c) BMT. Whole cell lysates were next obtained and protein concentrations determined with Pierce BCA Protein Assay (Thermo Scientific). Equal amounts of protein (20 µg) were separated by SDS-PAGE gel electrophoresis (120V, 1.5h) and subsequently transferred to polyvinylidene difluoride (PVDF) membrane using a Bio-Rad semi-dry transfer cell (Hercules, CA) (20V, 1h). The following antibodies were used to analyze the membranes with dilutions in accordance with the manufacturers specification sheet: α-tubulin (Cell Signaling, clone 11H10), Acetyl histone H4 (Lys5/8/12/16) (EMD Millipore, clone 3HH-4C10), SLC5A8 (abcam), GPR43 (abcam), Occludin (abcam, clone EPR8208), JAM (abcam, clone EP1042Y), and Claudin 5 (abcam). Secondary anti-rabbit antibody conjugated to HRP (Jackson ImmunoResearch, Cat No. 111-035-003) was used to detect primary antibodies, where needed. Densitometric analysis was performed using ImageJ software (National Institutes of Health; Bethesda, MA).
Mathewson N.D., Jenq R., Mathew A.V., Koenigsknecht M., Hanash A., Toubai T., Oravecz-Wilson K., Wu S.R., Sun Y., Rossi C., Fujiwara H., Byun J., Shono Y., Lindemans C., Calafiore M., Schmidt T.C., Honda K., Young V.B., Pennathur S., van den Brink M, & Reddy P. (2016). Gut microbiome derived metabolites modulate intestinal epithelial cell damage and mitigate Graft-versus-Host Disease. Nature immunology, 17(5), 505-513.
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