Immulite 2000 xpi immunoassay system

Manufactured by Siemens

Sourced in Germany, United States

The IMMULITE 2000 XPi Immunoassay System is a fully automated, random-access analyzer designed for processing various immunoassay tests. It is capable of performing a wide range of diagnostic tests, including those for hormones, special proteins, and infectious diseases.

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30 protocols using immulite 2000 xpi immunoassay system

Inflammatory Biomarkers in Cardiovascular Procedures

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Complete blood count and biochemistry will be evaluated at the central laboratory of the County Clinical Emergency Hospital of Tirgu Mures as per routine clinical practice, at baseline.
Inflammatory status will be assessed at baseline, respectively at 1-year post procedure. hsCRP levels will be assayed by immunoturbidimetric assay (COBAS INTEGRA 400 plus Analyser, Roche Diagnostics, Switzerland). Matrix metalloprotease 9 and interleukin-6 levels will be assessed via enzyme immunoassay (ELISA) method (Dynex DSX Automated ELISA System, Dynex Technologies, USA; IMMULITE 2000 XPi Immunoassay System, Siemens, USA). Determination of all inflammatory biomarkers will be performed at the Advanced Medical and Pharmaceutical Research Center of the University of Medicine and Pharmacy Targu-Mures. NT-proBNP serum levels will be determined prior to the procedure, at 24 hours, and repeated after 1 year, at the central laboratory of the Emergency Clinical County Hospital of Tirgu-Mures using an electrochemiluminescent immunoassay (IMMULITE 2000 XPi Immunoassay System, Siemens, USA).

Korodi S., Toganel R., Benedek T., Hodas R., Chitu M., Ratiu M., Kovacs I., Mester A, & Benedek I. (2019). Impact of inflammation-mediated myocardial fibrosis on the risk of recurrence after successful ablation of atrial fibrillation – the FIBRO-RISK study: Protocol for a non-randomized clinical trial. Medicine, 98(9), e14504.

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Biomarkers of Inflammatory Status

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At baseline, electrochemiluminescent immunoassay (IMMULITE 2000 XPi Immunoassay System, Siemens, United States of America), biochemistry, blood count and NT-proBNP will be determined. Also, inflammatory status will be determined and analyzed at the Advanced Medical and Pharmaceutical Research Center of the University of Medicine and Pharmacy Tirgu-Mures, Romania, based on serum high sensitive C-reactive protein levels determined using immunoturbidimetric assay (COBAS INTEGRA 400 plus analyzer, Roche Diagnostics, Switzerland).
MMP-9 and Il-6 levels will be also evaluated using ELISA method (Dynex DSX Automated ELISA System, Dynex Technologies; IMMULITE 2000 XPi Immunoassay System, Siemens, United States of America).^{[20 ]}

Stanescu A.G., Benedek I., Opincariu D., Hodas R., Ratiu M, & Benedek T. (2021). Assessment of lesion-associated myocardial ischemia based on fusion coronary CT imaging – the FUSE-HEART study: A protocol for non-randomized clinical trial. Medicine, 100(14), e25378.

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Biomarkers of Inflammatory Status

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Quantification of Serum and Salivary Cortisol

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Serum cortisol was measured in the clinical laboratory at Alder Hey Children's Hospital by immunoassay using the Siemens Immulite 2000XPi immunoassay system (Siemens Diagnostics, Camberley, Surrey), an automated immunoassay analyser using reagents supplied by the manufacturer. This assay has an intra-and inter-assay coefficient of variation of <5% and <7% respectively. LC-MS/MS analysis for salivary cortisone and cortisol was performed using a Waters Xevo TQS micro mass spectrometer and a Waters Acquity iclass LC system with an electrospray source operated in positive ionization mode following sample preparation by protein precipitation. The LOQ for both analytes is 0.3 nmol/L, with intraassay CV of <=3.3 nmol/L for cortisol, <=3.2 nmol/L for cortisone and interassay CV of <=4.5% for cortisol and <=3% for cortisone at concentrations 5-150 nmol/L.

Titman A., Price V., Hawcutt D., Chesters C., Ali M., Cacace G., Lancaster G.A., Peak M, & Blair J.C. (2020). Salivary cortisol, cortisone and serum cortisol concentrations are related to age and body mass index in healthy children and young people. Clinical endocrinology, 93(5).

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Diabetic Polyneuropathy Biomarkers Assessment

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Serum folic acid (normal range 3.9–16.9 ng/ml), Vitamin B12 (normal range 193–982 pg/ml) and homocysteine (normal range 5–15 micmol/l) levels were determined with automated immunoassay device (Immulite 2000 XPi Immunoassay System, Siemens Healthcare Diagnostics Inc., Tarrytown, NY, USA).
The NCS variables for diabetic polyneuropathy assessment were done by EMG and nerve conduction velocity (EMG-NCS). Lower limb NCS was determined because lower limb nerves are involved more than upper limb nerves.[8 (link)]

Mottaghi T., Khorvash F., Kheirollahi M., Maracy M, & Askari G. (2019). The MTHFR C677T polymorphism influences the efficacy of folic acid supplementation on the nerve conduction studies in patients with diabetic polyneuropathy; A randomized, double blind, placebo-controlled study. Journal of Research in Medical Sciences : The Official Journal of Isfahan University of Medical Sciences, 24, 36.

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Evaluating miR-107 Impact on CHO-hEPO Productivity

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To evaluate the impact of miR-107 overexpression on productivity, 2 × 10⁵ CHO-hEPO cells were transfected with the PB-miR-107-GFP vector (referred to as the miR-107 group) and with pb531-b1 (serving as a Mock) in a 6-well plate. Non-transfected CHO-hEPO cells (NT) were used as an additional control group. The cell culture supernatant from each group (miR-107, Mock, and NT) was collected, and the concentration of secreted EPO was measured.
EPO concentration was quantified using the Immulite EPN kit (L2KEPN2) produced by Siemens (Llanberis, Gwynedd, UK). The analysis was facilitated using the IMMULITE 2000 XPi immunoassay system, an automated chemiluminescent immunoassay analyzer, following the protocols outlined by the manufacturer (Siemens Healthineers, Germany). The specific productivity (pg/cell/day) was derived through the following Eq. 1, where CP (μg/ ml) represents the EPO concentration, and VCC (cells/mL) denotes the viable cell concentration (Maccani et al. 2014 (link)).

q_{p} = \frac{CP}{VCC} \times 10^{6} .

Jari M., Abdoli S., Bazi Z., Shamsabadi F.T., Roshanmehr F, & Shahbazi M. (2024). Enhancing protein production and growth in chinese hamster ovary cells through miR-107 overexpression. AMB Express, 14, 16.

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Monitoring IL-6 Levels in Trauma Care

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IL-6 [pg/ml] was measured with blood samples taken during stabilization in the emergency department after hospital admission. These samples represent the immunological response to trauma before emergency surgery was initiated (day 1). During the intensive care treatment, blood samples were taken repetitively every morning at 07:00 a.m. for at least 14 days. The IL-6 samples were part of the daily routine performed at the Center of Laboratory Medicine, Hannover Medical School, Hannover, Germany. Therefore, there was no need for special blood collection for this study. The measurement of IL-6 was performed with the IMMULITE 2000 XPi Immunoassay System©, Siemens AG©, Healthcare Sector, Erlangen, Germany.

Andruszkow H., Fischer J., Sasse M., Brunnemer U., Andruszkow J.H., Gänsslen A., Hildebrand F, & Frink M. (2014). Interleukin-6 as inflammatory marker referring to multiple organ dysfunction syndrome in severely injured children. Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine, 22, 16.

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Exercise-Induced Immune Response Profiling

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Blood samples were collected for the isolation of PBMCs and for detection of IL‐6, CK and CRP levels. Blood samples (30 ml) were drawn from the antecubital vein from each subject while seated pre‐, 5 min post‐ and 24 h post‐exercise to Vacutainer VACUETTE K3EDTA (Greiner Bio‐One) tubes containing anticoagulant (EDTA). Whole blood count and the number of lymphocytes and monocytes were assessed by Sysmex XP‐300 (Sysmex Corporation) to obtain the number of leukocytes and platelets. 18 ml of blood was used for isolation of PBMCs and 12 ml for serum isolation.
For the detection of IL‐6, CK and CRP, serum was isolated from blood samples by centrifugation (10 min 2200 g). Serum was stored in −20°C for later analysis, which was performed using IMMULITE 2000 XPi Immunoassay System (Siemens Healthcare GmbH) according to manufacturers´ instructions. Lactate was measured from fingertip blood samples, which were collected into capillary tubes (20 μl) at all three time points. Samples were then placed in a 1 ml hemolyzing solution and analyzed automatically with Biosen C‐line lactate analyzer (EKF diagnostic, Biosen).

Lähteenmäki E.I., Koski M., Koskela I., Lehtonen E., Kankaanpää A., Kainulainen H., Walker S, & Lehti M. (2022). Resistance exercise with different workloads have distinct effects on cellular respiration of peripheral blood mononuclear cells. Physiological Reports, 10(14), e15394.

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Quantifying Levosulpiride Adherence via Serum PRL

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PRL levels in serum confirmed adherence to levosulpiride treatment (also monitored by counting drug tablet return) and were quantified using the IMMULITE 2000 XPi immunoassay system (Siemens, Munich, Germany). The intra-assay and inter-assay coefficients of variation were less than 1%.

Núñez-Amaro C.D., López M., Adán-Castro E., Robles-Osorio M.L., García-Franco R., García-Roa M., Villalpando-Gómez Y., Ramírez-Neria P., Pineiro N., Rubio-Mijangos J.F., Sánchez J., Ramírez-Hernández G., Siqueiros-Márquez L., Díaz-Lezama N., López-Star E., Bertsch T., Marínez de la Escalera G., Triebel J, & Clapp C. (2023). Levosulpiride for the treatment of diabetic macular oedema: a phase 2 randomized clinical trial. Eye, 38(3), 520-528.

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Plasma Metabolite Profiling via GC-MS

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Blood samples were collected from the coccygeal vessel at 0900 h on d 18 of each period into 10 mL sodium heparin vacutainer vials (Becton Dickinson). Samples were kept on ice until plasma isolation. Plasma was isolated by centrifuging blood tubes at 1,500 × g for 10 Kristensen's (2000) (link) method for GC-MS analysis. In short, plasma was combined with acetonitrile and 2-chloroethanol before centrifuging at 1,500 × g for 30 min at -4°C. After centrifuging, the supernatant, NaOH, and heptane were combined to allow phase separation. The bottom phase was removed and transferred to a new vial for the addition of HCl, pyridine, chloroethyl chloroformate, and water. Lastly, chloroform was added to complete derivatization. Samples (0.80 μL) were analyzed on a Thermo Electron Focus gas chromatograph (Thermo Scientific) working with a Thermo Electron Polaris Q mass spectrometer (Thermo Scientific) and XCalibur software (version 1.4; Thermo Scientific). Plasma BHB concentrations were measured using a BHB (ketone body) colorimetric assay kit (Cayman Chemical Item No. 700190, Cayman Chemical). Plasma glucose concentrations were measured using Stanbio Glucose Liquid Reagent for Diagnostic Set (Fisher Scientific SB-1070-125). Plasma insulin concentrations were measured using a chemiluminescence assay (Siemens Immulite 2000 XPi Immunoassay System).

Beckett L., Gleason C.B., Bedford A., Liebe D., Yohe T.T., Hall M.B., Daniels K.M, & White R.R. (2021). Rumen volatile fatty acid molar proportions, rumen epithelial gene expression, and blood metabolite concentration responses to ruminally degradable starch and fiber supplies. Journal of dairy science, 104(8).

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