For the migration assay, cells were grown in 10% FCS medium. Normoxic control experiments were performed at 21% O2, and hyperoxic experiments were performed at 80% O2 conditions. Dimethyl sulfoxide (DMSO) was used as the solvent control as the steroids were dissolved in DMSO. Cells were treated with E2 (17β-estradiol; Sigma-Aldrich, Taufkirchen, Germany), DHEA (5-andostene-3b-ol-17-one; Sigma-Aldrich), DHEA+E2, 16α-OH-DHEA (5-androsten-3β, 16α-diol-17-one; Steraloids, Newport, RI), 16α-OH-DHEA+E2, adiol (5-androsten-3β, 17β-diol; Steraloids), and adiol+E2. All steroids were added to a final concentration of 100 nM.
Adiol
Manufactured by Steraloids
Adiol is a laboratory equipment product designed for use in scientific research and analysis. It functions as a precision measurement tool, enabling accurate quantification of specific chemical or biological components within a sample. The core purpose of Adiol is to facilitate analytical processes, providing researchers with reliable data to support their investigations.
Automatically generated - may contain errors
Lab products found in correlation
1 protocol using adiol
1
Steroid Effects on Cell Migration
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