Log In Sign up
The largest database of trusted experimental protocols

Human phospho mapk antibody array

Manufactured by R&D Systems
Visit Supplier
Sourced in United States

The Human phospho MAPK antibody array is a multiplex assay that allows for the simultaneous detection and quantification of the phosphorylation of multiple MAPK (Mitogen-Activated Protein Kinase) proteins in human samples. The array contains antibodies specific to various phosphorylated MAPK proteins, enabling the assessment of their activation status.

Automatically generated - may contain errors

Lab products found in correlation

Micro bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Irdye 800cw streptavidin, by LI COR (1 mentions) Odyssey clx imaging system, by LI COR (1 mentions)

Spelling variants of this product

Phospho-MAPK array (3 citations) MAPK) Antibody Array (2 citations)

5 protocols using human phospho mapk antibody array

1

Phospho-MAPK Antibody Array Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The phosphorylation of signaling mediators was analyzed using the Human Phospho‐MAPK Antibody Array (#ARY002B, R&D Systems) according to the manufacturer's protocol. Protein lysates were used from A375 and A2058 cells transduced with shSCR or shT28‐1 lentivirus. The HRP‐coupled streptavidin from the kit was replaced with IRdye 800CW Streptavidin (LI‐COR Biosciences), and all signals were analyzed using an Odyssey CLx Imaging System (LI‐COR Biosciences).
Nyberg W.A., Velasquez‐Pulgarin D.A., He T., Sjöstrand M., Pellé L., Covacu R, & Espinosa A. (2022). The bromodomain protein TRIM28 controls the balance between growth and invasiveness in melanoma. EMBO Reports, 24(1), e54944.
+ Open protocol
+ Expand
2

Phospho-MAPK Profiling in Cell Lysates

Check if the same lab product or an alternative is used in the 5 most similar protocols
The profile or protein phosphorylation in untreated and treated cell lysate was conducted by using Human Phospho-MAPK Antibody Array (R&D Systems, Minneapolis, MN, USA; cat. no. ARY002) following the manufacturer’s instructions. Protein spots were visualized using the chemiluminescence detection reagents supplied in the kit. The intensity score of each duplicated array spot was captured by C-DiGit blot scanner and analysed by Image Studio Software, and the averaged intensity was calculated by subtracting the averaged background signal. The fold change was obtained by comparing samples with the LPS-treated sample (set to 1).
Oddi S., Scipioni L., Totaro A., Angelucci C., Dufrusine B., Sabatucci A., Tortolani D., Coletta I., Alisi M.A., Polenzani L., Assfalg M., Caltagirone C., Dainese E, & Maccarrone M. (2019). The anti-inflammatory agent bindarit acts as a modulator of fatty acid-binding protein 4 in human monocytic cells. Scientific Reports, 9, 15155.
+ Open protocol
+ Expand
3

Phospho MAPK Antibody Array Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
TTA harvested at day 8 post-treatment and the tumor tissues harvested 24 hr post treatment were homogenized using a 21-gauge syringe in lysis buffer as described earlier 3 (link). Insoluble material were removed by centrifugation (15 min,12000g). The protein concentration was determined using micro BCA protein assay kit (Thermo scientific Pierce, Rockford, IL). 125 μg of lysate was hybridized to a human phospho MAPK antibody array (R&D Systems Inc, Minneapolis, MN) as instructed.
Sethi P., Jyoti A., Swindell E.P., Chan R., Langner U.W., Feddock J.M., Nagarajan R., O'Halloran T.V, & Upreti M. (2015). 3D Tumor tissue analogs and their orthotopic implants for understanding tumor-targeting of microenvironment-responsive nanosized chemotherapy and radiation. Nanomedicine : nanotechnology, biology, and medicine, 11(8), 2013-2023.
+ Open protocol
+ Expand
4

Phospho-MAPK Profiling of TES Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
TES harvested 10 days post-treatment were homogenized using a 21-gauge syringe in lysis buffer as described earlier28 (link). Insoluble material were removed by centrifugation (15 min, 12000 g). The protein concentration was determined using micro BCA protein assay kit (Thermo scientific Pierce, Rockford, IL). 125 μg of lysate was hybridized to a human phospho MAPK antibody array (R&D Systems Inc, Minneapolis, MN) as instructed.
Jyoti A., Fugit K.D., Sethi P., McGarry R.C., Anderson B.D, & Upreti M. (2015). An in vitro assessment of liposomal topotecan simulating metronomic chemotherapy in combination with radiation in tumor-endothelial spheroids. Scientific Reports, 5, 15236.
+ Open protocol
+ Expand
5

SCNN1B Impacts MAPK Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
MAPK signaling PCR array (Qiagen) was used to determine the effect of SCNN1B on gene expression. Phosphorylation status of MAPK proteins was investigated using human phospho-MAPK antibody array (R&D Systems).
Qian Y., Zhou L., Luk S.T., Xu J., Li W., Gou H., Chen H., Kang W., Yu J, & Wong C.C. (2022). The sodium channel subunit SCNN1B suppresses colorectal cancer via suppression of active c-Raf and MAPK signaling cascade. Oncogene, 42(8), 601-612.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!