An intravenous glucose tolerance test (IVGTT) was performed under full GA prior to the animal being rendered diabetic, after induction of diabetes, and post-transplant at monthly intervals. An intravenous bolus of dextrose (0.5 g/kg body weight) was injected into the cephalic venous line followed by a saline flush. Blood samples were obtained before injection and at 1, 2.5, 3, 5, 7.5, 10, 15, 20, 30, 40, 45, 50, 60, 75, 90, 105 and 120 min after injection. Blood glucose concentrations were measured using a dry chemistry method on a Vitros 5.1/FS system (Ortho-Clinical Diagnostics, Rochester, NY). NHP and porcine insulin were measured using Mercodia Insulin ELISA and c-peptide concentrations were measured using a Mercodia Porcine C-peptide ELISA (Mercodia AB, Uppsala, Sweden). K values [percentage decline of natural logarithm (blood glucose)/min] were calculated with blood glucose measurements from the IVGTT.
Vitros 5.1 fs system
Manufactured by Ortho Clinical Diagnostics
The Vitros 5.1/FS system is an automated clinical chemistry analyzer designed for in vitro diagnostic testing. It is capable of performing a variety of assays to measure different analytes in biological samples.
Automatically generated - may contain errors
2 protocols using vitros 5.1 fs system
1
Intravenous Glucose Tolerance Test for Diabetes
2
Comprehensive Metabolic Profiling Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum and plasma were prepared from collected venous blood samples (15 mL) and then
frozen (-80°C) for storage until analysis.
Insulin levels were determined by chemiluminescence and processed in the IMMULITE
2000 (Siemens Healthcare Diagnostics Products Ltd) analyzer, and glucose levels were
analyzed in the VITROS 5.1 FS system (Ortho Clinical Diagnostics, Johnson &
Johnson) with Micro SlideTM technology. HOMA-IR was calculated based on
the formula: HOMA-IR = insulin (mU/L) x glucose (mmol/L)/22.5, taking 3 as the cutoff
value for the diagnosis of insulin resistance.
Total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein
cholesterol and triglyceride levels were measured using an automated biochemical
VITROS 5.1 FS analyzer (Ortho Clinical Diagnostics, Johnson & Johnson).
Apolipoprotein A-I, B and lipoprotein (a) levels were determined by
immunonephelometry and processed in the BN ProSpec® System (Siemens Healthcare
Diagnostics Inc.) analyzer.
Leptin (eBioscience, San Diego, CA, USA) and adiponectin (BioVendor, Brno, Czech
Republic) were evaluated using commercially available enzyme-linked immunosorbent
assay kits, and the absorbances were measured at 450 nm (BIO-RAD Microplate Reader
Model 680, Hercules, CA, USA).
frozen (-80°C) for storage until analysis.
Insulin levels were determined by chemiluminescence and processed in the IMMULITE
2000 (Siemens Healthcare Diagnostics Products Ltd) analyzer, and glucose levels were
analyzed in the VITROS 5.1 FS system (Ortho Clinical Diagnostics, Johnson &
Johnson) with Micro SlideTM technology. HOMA-IR was calculated based on
the formula: HOMA-IR = insulin (mU/L) x glucose (mmol/L)/22.5, taking 3 as the cutoff
value for the diagnosis of insulin resistance.
Total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein
cholesterol and triglyceride levels were measured using an automated biochemical
VITROS 5.1 FS analyzer (Ortho Clinical Diagnostics, Johnson & Johnson).
Apolipoprotein A-I, B and lipoprotein (a) levels were determined by
immunonephelometry and processed in the BN ProSpec® System (Siemens Healthcare
Diagnostics Inc.) analyzer.
Leptin (eBioscience, San Diego, CA, USA) and adiponectin (BioVendor, Brno, Czech
Republic) were evaluated using commercially available enzyme-linked immunosorbent
assay kits, and the absorbances were measured at 450 nm (BIO-RAD Microplate Reader
Model 680, Hercules, CA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!