All oligonucleotides were synthesized and HPLC purified by Sangon Biotechnology Co. Ltd. (Shanghai, China). Thermostable Ampligase was obtained from Epicenter Technologies (Madison, WI, U.S.A.), exonuclease I (Exo I) and exonuclease III (Exo III) were purchased from New England Biolabs (Ipswich, MA, U.S.A.), magnesium chloride (MgCl2), ammonium sulfate (NH4)2SO4, bovine serum albumin (BSA), trolox, glucose oxidase, d -glucose, and catalase were obtained from Sigma-Aldrich Company (St. Louis, MO, U.S.A.). The streptavidin-coated quantum dots with the maximum emission at 605 nm (Qdot 605 ITK) were obtained from Life Technologies (Eugene, Oregon, U.S.A.). All other reagents were of analytical grade and used just as received without further purification. The ultrapure water was prepared by a Millipore filtration system (Millipore, Milford, MA, U.S.A.).
Thermostable ampligase
Manufactured by Illumina
Sourced in United States
Thermostable Ampligase is a highly thermostable DNA ligase enzyme that catalyzes the joining of DNA fragments with a high degree of efficiency and specificity. Its core function is to enable the ligation of DNA strands during various molecular biology techniques.
Automatically generated - may contain errors
Lab products found in correlation
D glucose, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Trolox, by Merck Group (1 mentions)
Glucose oxidase, by Merck Group (1 mentions)
Catalase, by Merck Group (1 mentions)
Millipore filtration system, by Merck Group (1 mentions)
Exonuclease 3 exo 3, by New England Biolabs (1 mentions)
Qdot 605 itk, by Thermo Fisher Scientific (1 mentions)
Exonuclease 1 exo 1, by New England Biolabs (1 mentions)
Bst dna polymerase large fragment, by New England Biolabs (1 mentions)
Betaine, by Merck Group (1 mentions)
Stepone real time pcr system, by Thermo Fisher Scientific (1 mentions)
T100 thermal cycler, by Bio-Rad (1 mentions)
2 protocols using thermostable ampligase
1
Quantum Dot-based Single-molecule Assay
2
Thermostable Ampligase-based DNA Detection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Thermostable Ampligase was purchased from Epicenter Technologies (USA). Bst DNA polymerase large fragment was obtained from New England Biolabs (USA). HPLC-purified DNA targets, probes and primers used in this study were purchased from Integrated DNA Technologies (USA). The betaine was obtained from Sigma-Aldrich (Shanghai, China). The dNTPs were purchased from Takara Biomedical Technology Co., Ltd. (Beijing, China). SYBR Green I (20 ng μL−1 stock solution in DMSO) was obtained from Zhishan Biotechnology Co., Ltd. (Xiamen, China). All the solutions were prepared with sterilized and deionized water. The sequences of oligonucleotides were listed in Table S1 (see in ESI† ).
The ligation reaction was carried out in a T100 Thermal Cycler (Bio-Rad, USA). The real-time fluorescence signal was monitored with the StepOne Real-Time PCR System (Applied Biosystems, USA).
The ligation reaction was carried out in a T100 Thermal Cycler (Bio-Rad, USA). The real-time fluorescence signal was monitored with the StepOne Real-Time PCR System (Applied Biosystems, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!