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Gpo pap reagent

Manufactured by Roche
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The GPO-PAP reagent is a laboratory product manufactured by Roche. It is used for the quantitative determination of glycerol in biological fluids. The reagent utilizes the glycerol phosphate oxidase-peroxidase (GPO-PAP) method to facilitate the enzymatic colorimetric measurement of glycerol.

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Lab products found in correlation

Pierce micro bca protein assay, by Thermo Fisher Scientific (2 mentions) Nefa c, by Fujifilm (1 mentions) Free glycerol reagent, by Merck Group (1 mentions) Ysi 2300, by Yellow Springs Instruments (1 mentions) Ultra sensitive mouse elisa, by Crystal Chem (1 mentions)

Close spelling variants of this product

Roche triglyceride reagent GPO-PAP TG GPO-PAP reagent GPO-PAP

4 protocols using gpo pap reagent

1

Measuring Adipocyte Lipid Profiles

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Concentration of non-esterified fatty acids (NEFA-C, WAKO Diagnostics, Richmond, VA, USA) and glycerol (Free glycerol reagent, Sigma-Aldrich, Castle Hill, NSW, Australia) was determined using commercial kits. Adipocyte triacylglycerol (TAG) content was extracted using the method of Folch et al. [26 (link)] and quantified using an enzymatic colorimetric method (GPO-PAP reagent, Roche Diagnostics). Cell protein content was determined using Pierce Micro BCA protein assay (Life Technologies Australia Pty Ltd., Scoresby VIC, Australia).
Balaban S., Shearer R.F., Lee L.S., van Geldermalsen M., Schreuder M., Shtein H.C., Cairns R., Thomas K.C., Fazakerley D.J., Grewal T., Holst J., Saunders D.N, & Hoy A.J. (2017). Adipocyte lipolysis links obesity to breast cancer growth: adipocyte-derived fatty acids drive breast cancer cell proliferation and migration. Cancer & Metabolism, 5, 1.
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2

Liver Lipid Content Quantification

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Liver tissue that was left over after creation of the liver cores was snap frozen and used to measure lipid content. The lipids in the liver slices were extracted overnight in chloroform:methanol (2:1). Triacylglycerol (TAG) content was determined by enzymatic colorimetric assay (GPO-PAP reagent, Roche Diagnostics) and expressed as µmol TAG/mg liver tissue.
Pasmans K., Adriaens M.E., Olinga P., Langen R., Rensen S.S., Schaap F.G., Olde Damink S.W., Caiment F., van Loon L.J., Blaak E.E, & Meex R.C. (2021). Hepatic Steatosis Contributes to the Development of Muscle Atrophy via Inter-Organ Crosstalk. Frontiers in Endocrinology, 12, 733625.
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3

Quantifying Glucose, Insulin, and Lipids

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Blood and plasma glucose levels were determined by an immobilized glucose oxidase method (YSI 2300; Yellow Springs Instruments, Yellow Springs, OH, USA). Plasma insulin was measured by ELISA (Ultra-Sensitive Mouse ELISA, Crystal Chem, USA). Liver triacylglycerols (TAGs) were extracted using the method of Folch [20 (link)], lipids were dried under N2 gas, resuspended in absolute ethanol and quantified using an enzymatic colorimetric method (GPO-PAP reagent; Roche Diagnostics).
Nagarajan S.R., Brandon A.E., McKenna J.A., Shtein H.C., Nguyen T.Q., Suryana E., Poronnik P., Cooney G.J., Saunders D.N, & Hoy A.J. (2017). Insulin and diet-induced changes in the ubiquitin-modified proteome of rat liver. PLoS ONE, 12(3), e0174431.
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4

Folch Extraction and Quantification

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Cell TAG were extracted using the method of Folch et al. (1957) and quantified using an enzymatic colorimetric method (GPO‐PAP reagent, Roche Diagnostics, North Ryde, NSW, Australia). Cell protein content was determined using Pierce Micro BCA protein assay (Life Technologies Australia Pty Ltd.).
Balaban S., Lee L.S., Varney B., Aishah A., Gao Q., Shearer R.F., Saunders D.N., Grewal T, & Hoy A.J. (2018). Heterogeneity of fatty acid metabolism in breast cancer cells underlies differential sensitivity to palmitate‐induced apoptosis. Molecular Oncology, 12(9), 1623-1638.
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