Vectastain abc rabbit igg kit

Manufactured by Vector Laboratories

Sourced in United States

The Vectastain ABC rabbit IgG kit is a reagent kit designed for the detection and visualization of rabbit immunoglobulin G (IgG) in various immunohistochemical and immunocytochemical applications. The kit provides the necessary components, including the ABC reagent and a chromogenic substrate, to facilitate the sensitive and specific detection of rabbit IgG in biological samples.

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Lab products found in correlation

Axiocam mrc, by Zeiss (2 mentions) Ba 1000, by Vector Laboratories (1 mentions) Chemmate envision, by Agilent Technologies (1 mentions) Hpa001888, by Atlas Antibodies (1 mentions) Bh 2 microscope, by Olympus (1 mentions) Blocking one reagent, by Nacalai Tesque (1 mentions) Dp73 camera, by Zeiss (1 mentions) Axiovert 200 microscope, by Zeiss (1 mentions) α gfap, by Agilent Technologies (1 mentions) Lsab hrp kit, by Agilent Technologies (1 mentions) Axioskop 2, by Zeiss (1 mentions) Axiovision software, by Zeiss (1 mentions) Dx51 microscope, by Olympus (1 mentions)

Close spelling variants of this product

Vectastain ABC-AP Rabbit IgG Kit VECTASTAIN Elite ABC Rabbit IgG Kit Vectastain Rabbit ABC kit Vectastain ABC kit ABC Rabbit IgG kit Vectastain ABC Vectastain kit ABC kit

6 protocols using vectastain abc rabbit igg kit

Histological Analysis of Skin Inflammation

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For the histological studies, one mouse in each treatment group was euthanized 3 weeks after the initiation of treatment. The posterior flank skin specimens were fixed in phosphate-buffered paraformaldehyde (4%), embedded in paraffin, and cut into 4 µm thick sections. The immunohistochemistry was performed using the Vectastain ABC rabbit IgG kit (Vector Laboratories, Inc., Burlingame, CA, USA) following the manufacturer's instructions. The following primary antibodies were used: anti-tumor necrosis factor (TNF)-α (NBP1-19532) polyclonal (Novus Biologicals, LLC, Littleton, CO, USA), anti-TXNIP rabbit polyclonal (Sigma-Aldrich; Merck KGaA), and anti-TRX (C63C6) rabbit monoclonal (Cell Signaling Technology, Inc., Danvers, MA, USA).
Intensity of staining were divided into four groups-no staining, weak staining, moderate staining and strong staining. One pathologist evaluated all pathological sections without the information of experimental design.

Hoshikawa H., Kamitori K., Indo K., Mori T., Kamata M., Takahashi T, & Tokuda M. (2018). Combined treatment with D-allose, docetaxel and radiation inhibits the tumor growth in an in vivo model of head and neck cancer. Oncology Letters, 15(3), 3422-3428.

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Immunohistochemical Detection of Rickettsia australis

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Murine tissues were collected immediately after euthanasia and fixed in 10% buffered formalin (1:10 tissue-formalin ratio). Samples were routinely processed and embedded in paraffin, and 5-μm sections were cut for hematoxylin and eosin (H&E) staining. Isolated tissues were additionally examined by immunohistochemistry to localize R. australis within the infected animals using anti-RcPFA, which recognizes several SFG rickettsial species (27 (link)). Primary antibody staining was followed by biotinylated anti-rabbit IgG secondary antibody (1:1,000, vector BA 1000; Vector Laboratories) and exposure to the detection reagent (Vectastain ABC rabbit IgG kit, vector PK 6102; Vector Laboratories). Slides were analyzed, micrographs captured, and pathological changes recorded by a Diplomate of the American College of Veterinary Pathologists (DACVP).

Riley S.P., Fish A.I., Del Piero F, & Martinez J.J. (2018). Immunity against the Obligate Intracellular Bacterial Pathogen Rickettsia australis Requires a Functional Complement System. Infection and Immunity, 86(6), e00139-18.

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Immunohistochemical Analysis of LRG Protein in Placental and Liver Tissues

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Human tissue 4-μm-thick sections (placenta and cord, fetal liver) were cut from paraffin blocks, then dewaxed and rehydrated. Rabbit antihuman LRG polyclonal antibody (HPA001888, Atlas Antibodies AB, Stockholm, Sweden, 1:1000) was used as the primary antibody and samples were visualized using EnVision ChemMate (Dako, Glostrup, Denmark) according to the manufacturer’s protocol. For analyses of mouse sections, dewaxed and rehydrated sections (4 μm) were incubated for 20 min in citrate buffer (10 mM citric acid, pH 6.0) at 95–100°C for antigen retrieval. Sections were then treated with 0.3% H₂O₂ and blocked using Blocking One reagent (Nacalai Tesque), followed by incubation with rabbit anti-mouse LRG1 polyclonal antibody (R322, Immuno-Biological Laboratories Co. Ltd., Gunma, Japan, 1:1,000) overnight at 4°C. After washing, sections were treated with the VECTASTAIN ABC Rabbit IgG Kit (Vector Laboratories, Burlingame, CA, USA). All sections were counterstained with hematoxylin.
Independent obstetricians (Fig 3B) [E.K. & Ki.Y. & S.M.], Fig 4C [E.K. & M.E. & Ka.Y.]), who were blinded to the histological data, analyzed the stained sections using an Olympus BH2 microscope for Figs 3B and 4C. The intensity of immunostaining was graded as 1 (weak staining), 2 (moderate), or 3 (strong). Grading was determined as the highest grade identified by sampling of 5–10 random fields for each tissue.

Kajimoto E., Endo M., Fujimoto M., Matsuzaki S., Fujii M., Yagi K., Kakigano A., Mimura K., Tomimatsu T., Serada S., Takeuchi M., Yoshino K., Ueda Y., Kimura T, & Naka T. (2020). Evaluation of leucine-rich alpha-2 glycoprotein as a biomarker of fetal infection. PLoS ONE, 15(11), e0242076.

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Muscle Histomorphometric Analysis

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The animals were sacrificed by cervical dislocation, and TA, extensor digitorum longus, soleus, and gastrocnemius (GA) muscles were dissected, weighed, and placed into 10% formalin for 2 days. Tissues were then processed for paraffin embedding and either H&E or trichrome stains (The OSU Pathology Core). Cross-sections of TA muscle were stained with anti-dystrophin to reveal myofiber boundaries. Muscle paraffin sections were deparaffinized, rehydrated, antigen-retrieved, incubated with anti-dystrophin (Spring Bioscience), and developed with the Vectastain ABC (rabbit IgG) kit (Vector Laboratories) followed by H&E counterstaining.
Morphometric analyses were performed on sections collected from similar region of each TA muscle. Ten images were captured from different areas covering the entire TA section with an Olympus DP73 camera attached to a Zeiss Axiovert 200 microscope. Images were analyzed with cellSens Digital Imaging software (Olympus Global) to determine the cross-sectional area from 500–1000 myofibers/animal.

Lin P.H., Duann P., Komazaki S., Park K.H., Li H., Sun M., Sermersheim M., Gumpper K., Parrington J., Galione A., Evans A.M., Zhu M.X, & Ma J. (2014). Lysosomal Two-pore Channel Subtype 2 (TPC2) Regulates Skeletal Muscle Autophagic Signaling. The Journal of Biological Chemistry, 290(6), 3377-3389.

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Immunohistochemical Staining of Mouse Brain

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Brains from mouse (postnatal day 19-21) were immediately fixed after dissection in 4% paraformaldehyde in 0.1 M phosphate buffer (PB), pH 7.4. Brains were cryoprotected in 30% sucrose in PB and stored at -80 °C till they were cut on a cryostat at 35 μm. Free-floating sections were stained with the indicated antibodies at 4°C overnight. Polyclonal rabbit α-PV (1:6000) and α-CR (1:4000) antibodies were from Swant, Bellinzona, Switzerland. Rabbit polyclonal α-GFAP (1:1500) antibody was from Dako, Hamburg, Germany and polyclonal rabbit α-TH (1:750) was from Abcam, Cambridge, UK. For these antibodies horseradish peroxidase and diaminobezidine substrate were used in conjunction with the Vectastain ABC Rabbit IgG Kit (Vector Laboratories, Burlingame, USA). Polyclonal goat α-ChAT (1:250) was from Millipore, Billerica, USA, and visualized with the DAKO LSAB™+/HRP kit. Photomicrographs were taken at a Zeiss Axioskop II equipped with AxioCam MRc and operated with Axiovision software (Carl Zeiss, Oberkochen, Germany).

Seeher S., Carlson B.A., Miniard A.C., Wirth E.K., Mahdi Y., Hatfield D.L., Driscoll D.M, & Schweizer U. (2014). Impaired selenoprotein expression in brain triggers striatal neuronal loss leading to coordination defects in mice. The Biochemical journal, 462(1), 67-75.

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Lymphoma Vascular Analysis Protocol

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All patients had biopsy-proven lymphoma. All tumour tissues were routinely fixed in 4% buffered formaldehyde and processed by standard methods into paraffin blocks, and 4-µm slides were prepared and stained with haematoxylin and eosin. A senior pathologist noted the number of vascular sections and the presence of capillary vascularisation via ERGmarker by field of view. For the ERG antibody (EP111, 1/100, pH 9; Epitomics, Burlingame, CA, USA), immunochemistry was performed with VECTASTAIN ABC Rabbit IgG Kit (Vector Laboratories, Burlingame, CA, USA). Samples were examined under an Olympus DX51 microscope (Olympus, Paris, France). All pathological samples were reviewed by one senior pathologist.

Tonnelet D., Bohn M.D., Becker S., Decazes P., Camus V., Thureau S., Tilly H., Jardin F, & Vera P. (2021). Angiogenesis imaging study using interim [18F] RGD-K5 PET/CT in patients with lymphoma undergoing chemotherapy: preliminary evidence. EJNMMI Research, 11, 37.

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