Rg1 (purity >95%) was purchased from Hongjiu Biotech Co., Ltd., (Tonghua, China). D-galactose (D-gal; purity >99%) was obtained from Sangon Biotech Co., Ltd. (Shanghai, China). The senescence-associated β-galactosidase (SA-β-gal) Staining kit was purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA), whereas the SOD, GSH-Px, GSH and MDA detection kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The AGEs (cat. no. abx512406) and 8-OH-dG (cat. no. SKT-120-480) kits were purchased from Shanghai Yuanye Biological Technology Co., Ltd. (Shanghai, China).
D galactose d gal
Manufactured by Sangon
Sourced in China
D-galactose (D-gal) is a monosaccharide sugar found in various natural sources. It is a core component of lactose, a disaccharide present in mammalian milk. D-gal is commonly used as a reference standard and reagent in biochemical and analytical applications.
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Lab products found in correlation
2 protocols using d galactose d gal
1
Aging Biomarker Quantification Protocol
2
Modeling Senescence in HEI-OC1 Cells
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HEI‐OC1 cells were cultured in high glucose Dulbecco's modified Eagle's medium (DMEM; Gibco, C12430500BT) containing 10% fetal bovine serum (FBS; Gibco, 10099141C) and 1% penicillin (Sangon Biotech, B540729) in a 33°C incubator with a CO2 concentration of 10%.
D‐galactose (D‐gal; Sangon Biotech, A600215) was dissolved in complete medium and filtered to achieve concentrations of 20, 40, and 60 mg/mL. The HEI‐OC1 cells were treated with these concentrations for 24 h to establish the senescence model. MYLS22 (MedChemExpresss, HY‐136446), Tyrphostin A9 (MedChemExpresss, HY‐15511), and kaempferol (MedChemExpresss, HY‐14590) were dissolved in dimethyl sulfoxide (DMSO) and diluted to final concentrations with complete medium. The MYLS22 group was pretreated with 10 μM for 6 h, followed by treatment with D‐gal (40 mg/mL) for 24 h. The Tyrphostin A9 group was pretreated with 5 μM for 6 h, followed by treatment with D‐gal (40 mg/mL) for 24 h. The kaempferol group was pretreated with 5 μM for 6 h and then co‐treated with D‐gal (40 mg/mL) for 24 h.
D‐galactose (D‐gal; Sangon Biotech, A600215) was dissolved in complete medium and filtered to achieve concentrations of 20, 40, and 60 mg/mL. The HEI‐OC1 cells were treated with these concentrations for 24 h to establish the senescence model. MYLS22 (MedChemExpresss, HY‐136446), Tyrphostin A9 (MedChemExpresss, HY‐15511), and kaempferol (MedChemExpresss, HY‐14590) were dissolved in dimethyl sulfoxide (DMSO) and diluted to final concentrations with complete medium. The MYLS22 group was pretreated with 10 μM for 6 h, followed by treatment with D‐gal (40 mg/mL) for 24 h. The Tyrphostin A9 group was pretreated with 5 μM for 6 h, followed by treatment with D‐gal (40 mg/mL) for 24 h. The kaempferol group was pretreated with 5 μM for 6 h and then co‐treated with D‐gal (40 mg/mL) for 24 h.
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