Gingival fibroblasts exposed to reconstituted casein and whey powder for 24 h were incubated with anti-smad2/3 antibody (D7G7 XP® Rabbit mAb, Cell Signaling, Danvers, MA, USA) and with Ki67 (8D5, Mouse mAb antibody, 9449 Cell Signaling Danvers, MA, USA) for 24 h at 4 °C. Following blocking by 1% BSA and permeabilization with 0.1% Triton X, an Alexa Fluor® 488-conjugated secondary antibody (Cell Signaling) was added for 1 h at room temperature. Images were captured under a fluorescent microscope (Axio Imager M2, Carl Zeiss AG, Oberkochen, Germany).
D7g7 xp rabbit mab
Manufactured by Cell Signaling Technology
Sourced in United States
D7G7 XP® Rabbit mAb is a laboratory reagent that can be used for the detection and analysis of targeted proteins. It is a monoclonal antibody produced in rabbit cells. The core function of this product is to specifically bind and identify the target protein in various experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using d7g7 xp rabbit mab
1
Gingival Fibroblast Proliferation Assay
2
Investigating Smad2/3 Signaling in Gingival Fibroblasts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Gingival fibroblasts exposed to human milk for 24 h were incubated with anti-Smad2/3 antibody (D7G7 XP® Rabbit mAb, Cell Signalling Danvers, MA) and for overnight at 4 °C. Following blocking by 1% BSA and permeabilization with 0.1% Triton X, an Alexa Fluor® 488-conjugated secondary antibody (Cell Signalling) was added for 1 h at room temperature. Images were captured under a fluorescent microscope (Axio Imager M2, Carl Zeiss AG, Oberkochen, Germany).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!