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Mouse il 1β elisa

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The Mouse IL-1β ELISA is a quantitative sandwich enzyme-linked immunosorbent assay designed to measure the concentration of Mouse Interleukin-1 Beta (IL-1β) in cell culture supernatants, serum, and plasma.

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Lab products found in correlation

Cba flex set, by BD (1 mentions) Facscanto 2, by Tree Star (1 mentions) Cytometric bead array cba mouse inflammation kit, by BD (1 mentions)

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IL-1β (109 citations) Mouse IL-1β (6 citations) ELISAs (6 citations)

2 protocols using mouse il 1β elisa

1

Cytokine Analysis in BAL Samples

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The CBA flex set (BD Bioscience) was used as per the manufacturer's instructions to determine the cytokine concentrations of BAL supernatant. Data were acquired using FACSCanto II and analyzed with FlowJo X version 10.0.7r2 (Tree Star) with sample concentrations interpreted from standard curves using GraphPad Prism 6.0 F. For the detection of IL-1β in the BAL, 50 μl undiluted sample supernatant was used in a mouse IL-1β ELISA (BD Biosciences) according to the manufacturer's specifications.
Lee P.(., Bird N., MacKenzie-Kludas C., Mansell A., Kedzierska K., Brown L, & McAuley J. (2016). Induction of memory cytotoxic T cells to influenza A virus and subsequent viral clearance is not modulated by PB1-F2-dependent inflammasome activation. Immunology and Cell Biology, 94(5), 439-446.
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2

Cytokine Profile Quantification

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IL-6, TNF-α, MCP-1, IL-12, IFN-γ and IL-10 detection was performed using the BD Cytometric Bead Array (CBA) Mouse Inflammation Kit (BD Bioscience). IL-1β and IL-17 quantification was performed using the Mouse IL-1β ELISA (BD Bioscience) and Mouse IL-17 Duo3.2.43 ELISA (R&D Systems) kits, respectively. All assays were performed according to the manufacturer’s instructions. The results are expressed as pg/mL.
Silva-de-França F., Villas-Boas I.M., Serrano S.M., Cogliati B., Chudzinski S.A., Lopes P.H., Kitano E.S., Okamoto C.K, & Tambourgi D.V. (2019). Naja annulifera Snake: New insights into the venom components and pathogenesis of envenomation. PLoS Neglected Tropical Diseases, 13(1), e0007017.
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