Inos ab178945

Curcumin was obtained from Selleck Chemicals (Houston, TX, USA) and dissolved in dimethyl sulfoxide (DMSO) to obtain an initial concentration of 10 mM and stored at −80°C. Phorbol-12-myristate-13-acetate (PMA) and lipopolysaccharide (LPS) were bought from Sigma-Aldrich (St. Louis, MO, USA). An Annexin V-APC/7-AAD kit was bought from Keygen Biotechnology (Nanjing, China). Rabbit antibodies against zonula occludens- (ZO-) 1 (8193), claudin-1 (13255), γH2AX (Ser139; 9718), glucose-regulated protein 78 (GRP78; 3183), protein kinase-like endoplasmic reticulum kinase (PERK; 5683), eukaryotic translation initiation factor 2α (eIF2α; 5324), p-eIF2α (3398), activating transcription factor 4 (ATF4; 11815), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; 2118) were purchased from Cell Signaling Technology (Danvers, MA, USA). Rabbit antibodies inducible nitric oxide synthase (iNOS; ab178945), C/EBP homologous protein (CHOP; ab179823), and caspase-12 (ab62484) were obtained from Abcam (Cambridge, UK). HRP-labelled goat anti-rabbit secondary antibody (HA1001) was purchased from HuaBio (Hangzhou, China).

A CCK-8 assay kit was purchased from Dojindo Molecular Technologies (Kumamoto, Japan). AST, ALT, BUN, and SCr test kits were supported by the Nanjing Jiancheng Bioengineering Institute (Nanjing, China). TNF-α and IL-6 ELISA kits were purchased from Elabscience Biotechnology (Houston, Texas, United States). A TUNEL assay kit (C10617) was purchased from Thermo Fisher Scientific (Carlsbad, CA, United States). The ROS assay kit and Annexin V-FITC Apoptosis Detection Kit were purchased from Beyotime Biotechnology (Shanghai, China). COX-2 antibody (CST, 12282S) and F4/80 antibody (70076S) were purchased from Cell Signaling Technology (Danvers, MA, United States). BAX (ab32503) and iNOS (ab178945) were obtained from Abcam (Chicago, IL, United States). Bcl-2 antibody (BF9103) and beta-actin (AF7018) were obtained from Affinity Bioscience (Jiangsu, China). The Cyt c antibody (d10933-1-AP) was purchased from Proteintech (Rosemont, IL, United States). Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed secondary antibody Alexa Fluor 488 (A11034) and Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed secondary antibody Alexa Fluor 555 (A21428) were obtained from Thermo Fisher Scientific (Carlsbad, CA, United States).