Citric acid content assay kit

The frozen brain samples and primary microglia after LPS/vehicle and/or hesperidin administration were used for citrate levels and Cs enzyme activity detection by Citric Acid Content Assay Kit (Solarbio, BC2155) and Citrate Synthase Activity Assay Kit (Solarbio, BC1065). The corresponding users' guide were followed. The data of Cs enzyme activity were normalized by the total protein per sample.

A total of 5 x 10⁶ cells were harvested and the concentration. of citrate was determined using a Citric Acid Content Assay Kit (Solarbio, BC2155, China). The citrate content (μmol/10⁴ cells) was calculated as follows: citrate content (μmol/10⁴ cells) = 2 × ΔA (sample - blank)/ ΔA (standard - blank)/ cell count. Similarly, the α-ketoglutarate (α-KG) content was measured using a α-Ketoglutaric Acid (α-KG) Content Assay Kit (Solarbio, BC5425, China) according to the following formula: α-KG content (nmol/10⁶ cells) = 475 × ΔA (sample - blank)/ ΔA (standard - blank)/ cell count × sample dilution factor.