The detail of the fabrication of the polydimethylsiloxane (PDMS) devices used can be found in our previous paper.20 (link) Briefly, the software “CleWin” was used for the design. An MW2 laser direct-write laser system (Durham Magneto Optics Ltd, Durham, UK) was used to pattern the SU8 (thickness of 25 μm) creating an SU8 on silicon master. A PDMS (PDMS monomer and curing agent used in a weight ratio of 10 : 1) copy of this silicon master was cross-linked at 75 °C for 1 h. Afterwards, access holes were punched into the PDMS copy, which was then given O2 plasma treatment (100 W, O2 pressure 0.5 mbar, 1 min, Zepto Plasma Unit, Diener Electronic, Germany) and bonded onto a glass slide. Post-bonding baking in an oven at 75 °C for 30 min increased the bonding strength and resulted in a device ready for use. For the flow focus design used in our experiments, the width of nozzle is about 15 μm and the width and length of channel after the nozzle are 100 and 4000 μm, respectively. The depth of all the channels is 25 μm.
Zepto plasma unit
Manufactured by Diener Electronic
Sourced in Germany
The Zepto Plasma Unit is a lab equipment designed for the generation and control of plasma. It provides a stable and adjustable plasma environment for various applications in research and industrial settings.
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Lab products found in correlation
2 protocols using zepto plasma unit
1
Fabrication of PDMS Microfluidic Devices
2
Fabrication of PDMS Microfluidic Channels
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PDMS channels were fabricated by pouring PDMS onto an aluminum mold and curing at 70 °C for 2 h. Fig. 1C shows the cross-section of the channel. It consists of three 23 mm long parallel sections. The two side sections are each 5 mm wide, and 1 mm high, and the central section is 7 mm wide and 500 μm high. There is no physical barrier between the three sections. The shallowest part of the microfluidic channel is located above the template, namely, the region of the channel floor bearing the microfabricated traps. The two parts of the microfluidic channel (ceiling and floor) can be plasma bonded (40 s plasma treatment with a Zepto Plasma Unit, Diener electronic GmbH), or held together using external clamps. Both sealing strategies were successfully tested.
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