Rabbit anti glial fibrillary acidic protein

NPCs were plated onto poly-L-lysine-coated 24-well culture dishes (150 000 cells per well) and cultured in medium supplemented with 10% fetal bovine serum (FBS). After 7 days in culture, immunocytochemical staining was performed as described in the Methods section. The following primary antibodies were used during an overnight incubation at 4°C: mouse anti-βIII tubulin (1:500; Sigma) and rabbit anti-glial fibrillary acidic protein (1:500; Santa Cruz Biotechnology). An Alexa Fluor 488-conjugated goat anti-mouse IgG antibody and an Alexa Fluor 546–conjugated goat anti-rabbit IgG antibody (Invitrogen) were each used at a dilution of 1:3 000. The number of immunoreactive cells in each well was counted using fluorescent microscopy in four independent fields.