Polyclonal rat anti f4 80 antibody

Double-labeled immunofluorescence was performed to localize RNase 6 expression in the human and murine urinary tract. Human kidney slides were incubated overnight at 4°C with a polyclonal rabbit RNase 6 antibody (1:150, Abcam), and an anti-CD68 antibody (1:200, Abgent, San Diego, CA, USA). Mouse kidney and bladder slides were incubated overnight at 4°C with a polyclonal rabbit RNase 6 antibody (1:150, Abcam), polyclonal rat Ly6g antibody (1:200, Biolegend, San Diego, CA), polyclonal rat anti-CD68 antibody (1:100, AbD Serotec, Raleigh, NC, USA), polyclonal rat anti-F4/80 antibody (1:100, AbD Serotec), or a biotinylated hamster anti-CD11c antibody (1:500, BD Pharmingen, San Jose, CA, USA). Reactivity was visualized using biotinylated or fluorophore conjugated secondary antibodies. Negative-control sections were incubated with non-immune serum in place of RNase 6 and RNase 7 antibodies. Images were obtained using the BZ-9000 series fluorescence microscope (KEYENCE, Osaka, Japan).