Buffer set 3

Manufactured by Macherey-Nagel

Sourced in Germany

Buffer Set III is a collection of buffers designed for various laboratory applications. The set includes a range of different buffer solutions with varying pH levels, allowing for the appropriate buffer selection for specific experimental requirements. This product provides a convenient and standardized solution for maintaining optimal pH conditions in various laboratory procedures.

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Lab products found in correlation

Simplinano spectrophotometer, by Harvard Bioscience (1 mentions) Nucleobond axg column, by Macherey-Nagel (1 mentions)

Close spelling variants of this product

Nucleobond® buffer set III Nucleobond Buffer set III kit

2 protocols using buffer set 3

Whole-Genome Sequencing and Variant Calling for S. pombe

Cited 2 times

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Cells were grown in YES liquid medium to exponential phase at 30°C and harvested by centrifugation. Genomic DNA was extracted and purified using NucleoBond AXG and Buffer Set III (MACHEREY-NAGEL) according to the manufacturer’s protocol. Whole-genome sequencing was performed by BGI JAPAN using a DNBseq platform, and the sequencing reads were mapped to the S. pombe reference genome using BWA⁸⁵ (link) and samtools,⁸⁶ (link) followed by variant calling using GATK HaplotypeCaller.⁹⁵ (link)^,⁹⁶ The mutations specifically found in the mutant strains were extracted and annotated using bcftools⁸⁷ (link) and SnpEff,⁸⁸ (link) respectively.

Morozumi Y., Mahayot F., Nakase Y., Soong J.X., Yamawaki S., Sofyantoro F., Imabata Y., Oda A.H., Tamura M., Kofuji S., Akikusa Y., Shibatani A., Ohta K, & Shiozaki K. (2023). Rapamycin-sensitive mechanisms confine the growth of fission yeast below the temperatures detrimental to cell physiology. iScience, 27(1), 108777.

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Genomic DNA Extraction for LAB Strains

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Genomic DNA from the LAB strains was extracted using a NucleoBond AXG column and Buffer Set III (U0544A and U0603A; MACHEREY-NAGEL, Düren, Germany) according to the manufacturer’s protocol. The DNA concentration was measured using a SimpliNano spectrophotometer (29061712; Biochrom, Cambridge, UK) and used for lipofection.

Sugimoto A., Numaguchi T., Chihama R., Takenaka Y, & Sato Y. (2023). Identification of novel lactic acid bacteria with enhanced protective effects against influenza virus. PLOS ONE, 18(8), e0273604.

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