Log In Sign up
The largest database of trusted experimental protocols

Anti flag m2 beads

Manufactured by Thermo Fisher Scientific
Visit Supplier

Anti-FLAG M2 beads are affinity beads designed for the purification and detection of FLAG-tagged proteins. They are composed of agarose beads covalently coupled with the anti-FLAG M2 monoclonal antibody, which specifically binds to the FLAG peptide tag. The beads enable efficient and selective capture of FLAG-tagged proteins from complex samples.

Automatically generated - may contain errors

Lab products found in correlation

Dynabead, by Thermo Fisher Scientific (2 mentions) Ip lysis buffer, by Thermo Fisher Scientific (2 mentions) Protease and phosphatase inhibitor cocktail, by Thermo Fisher Scientific (2 mentions) Tris acetate gel, by Thermo Fisher Scientific (2 mentions) Anti flag m2 magnetic bead, by Merck Group (2 mentions) Nupage 10 bis tris, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

Anti-FLAG (43 citations) Anti-FLAG beads (5 citations) Anti-FLAG M2 (4 citations) Flag M2 beads (2 citations) Flag-beads (2 citations)

4 protocols using anti flag m2 beads

1

Immunoprecipitation and Western Blotting

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in IP lysis buffer (Thermo Fisher Scientific, 87787) with protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific, 78440) for immunoprecipitation and Western blotting. Dynabeads (Thermo Fisher Scientific, 88847) preincubated with antibody or anti-FLAG M2 beads (Thermo Fisher Scientific, A36797) were used to immunoprecipitate proteins of interest based on the manufacturer's protocol. Antibodies used in immunoprecipitation and Western blot are as follows: TUBULIN (Sigma-Aldrich, T9026, RRID:AB_477593), ITCH (BD, 611198, RRID:AB_398732), HA (CST, 3724S, RRID:AB_1549585), FLAG (CST, 14793S, RRID:AB_2572291), PD-L1 (CST, 13684S, RRID:AB_2687655), GAPDH (CST, 5174S, RRID:AB_10622025), and UBIQUITIN (CST, 3933S, RRID:AB_2180538; CST, 3936S, RRID:AB_331292).
Yang Z., Wang Y., Liu S., Deng W., Lomeli S.H., Moriceau G., Wohlschlegel J., Piva M, & Lo R.S. (2022). Enhancing PD-L1 Degradation by ITCH during MAPK Inhibitor Therapy Suppresses Acquired Resistance. Cancer Discovery, 12(8), 1942-1959.
+ Open protocol
+ Expand
2

Protein Extraction and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in RIPA buffer for western blot analysis or in RIPA IP buffer for co-immunoprecipitation (Co-IP). Anti-FLAG M2 Magnetic beads (Sigma-Aldrich, MO) eluted FLAG-tagged proteins. Total protein lysates or eluates of the anti-FLAG M2 beads were resolved on NuPAGE 10% Bis-Tris or 3–8% Tris-acetate gels (Thermo Fisher Scientific, MA).
Sun N., Nasello C., Deng L., Wang N., Zhang Y., Xu Z., Song Z., Kwan K., King R.A., Pang Z.P., Xing J., Heiman G.A, & Tischfield J.A. (2017). The PNKD gene is associated with Tourette Disorder or Tic disorder in a multiplex family. Molecular psychiatry, 23(6), 1487-1495.
+ Open protocol
+ Expand
3

Protein Extraction and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in RIPA buffer for western blot analysis or in RIPA IP buffer for co-immunoprecipitation (Co-IP). Anti-FLAG M2 Magnetic beads (Sigma-Aldrich, MO) eluted FLAG-tagged proteins. Total protein lysates or eluates of the anti-FLAG M2 beads were resolved on NuPAGE 10% Bis-Tris or 3–8% Tris-acetate gels (Thermo Fisher Scientific, MA).
Sun N., Nasello C., Deng L., Wang N., Zhang Y., Xu Z., Song Z., Kwan K., King R.A., Pang Z.P., Xing J., Heiman G.A, & Tischfield J.A. (2017). The PNKD gene is associated with Tourette Disorder or Tic disorder in a multiplex family. Molecular psychiatry, 23(6), 1487-1495.
+ Open protocol
+ Expand
4

Protein Immunoprecipitation and Western Blotting

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in IP lysis buffer (Thermo Fisher Scientific, 87787) with protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific, 78440) for immunoprecipitation (IP) and Western blotting. Dynabeads (Thermo Fisher Scientific, 88847) pre-incubated with antibody or anti-FLAG M2 beads (Thermo Fisher Scientific, A36797) were used to immunoprecipitate proteins of interest based on the manufacturer’s protocol. Antibodies used in IP and Western blot are as follows: TUBULIN (Sigma Aldrich, T9026, RRID:AB_477593), ITCH (BD, 611198, RRID:AB_398732), HA (CST, 3724S, RRID:AB_1549585), FLAG (CST, 14793S, RRID:AB_2572291), PD-L1 (CST, 13684S, RRID:AB_2687655), GAPDH (CST, 5174S, RRID:AB_10622025), UBIQUITIN (CST, 3933S, RRID:AB_2180538; CST, 3936S, RRID:AB_331292).
Yang Z., Wang Y., Liu S., Deng W., Lomeli S.H., Moriceau G., Wohlschlegel J., Piva M, & Lo R.S. (2022). Enhancing PD-L1 Degradation by ITCH during MAPK Inhibitor Therapy Suppresses Acquired Resistance. Cancer Discovery, 12(8), 1942-1959.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!