Chronic in vivo recordings were conducted during 20 minute forelimb reach training sessions before, during, and after cuprizone treatment. A single 1.6mm vertical NeuroNexus recording electrode was chronically inserted into primary motor cortex (300μm anterior to bregma, 1.5mm lateral bregma) contralateral to the trained forelimb. Data was recorded using Cheetah acquisition software at 30kHz (NeuroNexus), and single unit activity was clustered using Spike Sort 3D (Neuralynx). Isolation Distance and L-Ratio was used to quantify cluster quality and noise contamination81. Spike data was binned at 10ms and trial-averaged. Heatmaps report average firing rate during 500 ms time window when the animal was not engaged in reach behavior.
Cheetah acquisition software
Manufactured by NeuroNexus
Cheetah acquisition software is a data acquisition and analysis platform used to record and process physiological signals from various sources. The software provides real-time data acquisition, display, and storage capabilities, enabling researchers to capture and analyze neural, behavioral, and other relevant data.
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Lab products found in correlation
2 protocols using cheetah acquisition software
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Chronic Motor Cortex Recordings During Cuprizone
2
Chronic Motor Cortex Recordings During Cuprizone
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Chronic in vivo recordings were conducted during 20 minute forelimb reach training sessions before, during, and after cuprizone treatment. A single 1.6mm vertical NeuroNexus recording electrode was chronically inserted into primary motor cortex (300μm anterior to bregma, 1.5mm lateral bregma) contralateral to the trained forelimb. Data was recorded using Cheetah acquisition software at 30kHz (NeuroNexus), and single unit activity was clustered using Spike Sort 3D (Neuralynx). Isolation Distance and L-Ratio was used to quantify cluster quality and noise contamination81. Spike data was binned at 10ms and trial-averaged. Heatmaps report average firing rate during 500 ms time window when the animal was not engaged in reach behavior.
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