Ab133357

Manufactured by Cell Signaling Technology

Sourced in United States

Ab133357 is a laboratory reagent used for detection and analysis in various experimental techniques. It is a high-quality antibody produced by Cell Signaling Technology. The core function of this product is to serve as a specific molecular probe for the detection and study of target proteins or other biomolecules.

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Lab products found in correlation

Rabbit anti phospho histone h2a x, by Cell Signaling Technology (1 mentions) Rabbit anti phospho histone h3, by Cell Signaling Technology (1 mentions) Ab183685, by Abcam (1 mentions) Ab213524, by Abcam (1 mentions) Ab213524, by Proteintech (1 mentions) Rabbit anti ki67, by Cell Signaling Technology (1 mentions)

2 protocols using ab133357

Antibody-based Immunostaining and Immunoblotting

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Rabbit anti-CMTM6 (Sigma-Aldrich; HPA026980), rabbit anti-PD-L1 (Abcam, USA; ab213524), rabbit anti-CD4 (Abcam; ab183685), rabbit anti-CD8 alpha (Abcam; ab217344), rabbit anti-CD68 (Proteintech; 28058-1-AP), rabbit anti-CD11b (Abcam; ab133357), and rabbit anti-Ki-67 (Cell Signaling Technology; #9129) were used for immunostaining.
Rabbit anti-CMTM6 (Sigma-Aldrich, HPA026980), rabbit anti-PD-L1 (Abcam; ab213524), rabbit anti-E-cadherin (Proteintech; 20874-1-AP), rabbit anti-N-cadherin (Proteintech; 22018-1-AP), rabbit anti-Snail (Proteintech; 13099-1-AP), rabbit anti-p53 (Proteintech; 10442-1-AP), rabbit anti-cyclin B1 (Proteintech; 55004-1-AP), and rabbit anti-phospho-Histone H2A.X (Cell Signaling Technology; #9718) were used for immunoblotting.
Rabbit anti-CMTM6 (Sigma-Aldrich, HPA026980), rabbit anti-phospho-Histone H2A.X (Cell Signaling Technology; #9718) and rabbit anti-phospho-histone H3 (Cell Signaling Technology; #53348) were used for immunofluorescence.

Wang H., Fan Y., Chen W., Lv Z., Wu S., Xuan Y., Wang C., Lu Y., Guo T., Shen D., Zhang F., Huang Q., Gao Y., Li H., Ma X., Wang B., Huang Y, & Zhang X. (2022). Loss of CMTM6 promotes DNA damage-induced cellular senescence and antitumor immunity. Oncoimmunology, 11(1), 2011673.

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Immunohistochemical Analysis of Tumor Immune Cells

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Briefly, tissues from the tumor areas were fixed with 4% paraformaldehyde at RT; dehydrated in 70%, 80%, 90%, and 100% ethanol (v/v); cleaned in xylene; and embedded in paraffin. Tissue sections (5 μm) following the antigen restoration were incubated with 3% hydrogen peroxide (H₂O₂) solution at RT for 10 minutes, immunostaining with CD11b (1:100, Abcam, ab133357), CD206 (1:500, Cell Signaling technology, 24595S) or CD86 (1:50, Cell Signaling technology, 91882S) at 4°C overnight. Development of section was performed with 3,3'-Diaminobenzidine (DAB, Abcam) and using haematoxylin as counterstain. Data were analyzed using the image acquisition and analysis system.

Tian L., Li H., Zhao P., Liu Y., Lu Y., Zhong R., Jin Y., Tan T, & Cheng Y. (2023). C-Myc-induced hypersialylation of small cell lung cancer facilitates pro-tumoral phenotypes of macrophages. iScience, 26(10), 107771.

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