Prism 6.0f for mac

The primary dependent measures were (1) percent cocaine-appropriate responding (% CAR) (defined as [number of responses on the cocaine-associated key divided by the total number of responses on both the cocaine-and saline-associated keys]*100), and (2) rates of responding in responses per second. % CAR was included in the analysis only if at least one ratio requirement was completed. These dependent measures were then plotted as a function of drug dose or time after test drug administration. Dose effects were analyzed using one-way ANOVA (Prism 6.0f for Mac, GraphPad, La Jolla, CA), and in the presence of a significant dose effect, a Dunnett post-hoc test was conducted to determine differences compared to saline. In addition, the effective drug dose to produce 50% CAR (ED₅₀) in dose-effect studies, and time for % CAR to decline to 50% (T₅₀) in time-course studies, were determined in each rat by either linear regression when at least three data points were available or interpolation when only two data points were available (one below and one above 50% CAR). Individual cocaine, phendimetrazine, and phenmetrazine ED₅₀ and T₅₀ values were then averaged to yield mean values and 95% confidence limits. Mean cocaine, phendimetrazine, and phenmetrazine ED₅₀ and T₅₀ values were compared by one-way ANOVA.

Data are expressed as mean ± SEM. To compare serial echocardiographic parameters and blood pressure measurements, we used a two-way ANOVA for repeated measurements. If the interaction of time and genotype was significant, unpaired Student's t-tests were used to compare parameters between genotypes. For the analysis of changes over time, paired Student t-tests were used. To compare cardiac biomarkers and molecular biology parameters, one- or two-way ANOVA were used, as appropriate. Linear regression analysis was used to determine the correlation coefficient (r²) between BAT activation estimated by UCP1 mRNA levels and LVEF after MI. In all experiments, P < 0.05 was considered significant. The analyses were performed using Prism 6.0f for Mac (GraphPad Software, La Jolla, California, USA, www.graphpad.com).