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Sirnas for mrnas

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SiRNAs for mRNAs are synthetic, double-stranded RNA molecules designed to temporarily silence the expression of specific messenger RNA (mRNA) targets. They function by binding to and degrading the target mRNA, thereby preventing the production of the corresponding protein.

Automatically generated - may contain errors

Lab products found in correlation

Amaxa nhdf nucleofector kit, by Lonza (2 mentions) On targetplus sirna, by Horizon Discovery (2 mentions)

2 protocols using sirnas for mrnas

1

Knockdown of DINO in Lung Fibroblasts

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Human fetal lung fibroblasts were transfected with 3 independent 50 nM ON-TARGETPlus siRNAs (Dharmacon) or 100nM ASOs40 (link) targeting DINO using the Lipofectamine 2000 reagent. Large scale transfections were performed using the Amaxa Nucleofector NHDF kit. siRNAs for mRNAs (Ambion) were used as a pool of two. U2OS cells were infected with shRNAs targeting DINO using the pGIPZ shRNAmir lentiviral system.
Schmitt A.M., Garcia J.T., Hung T., Flynn R.A., Shen Y., Qu K., Payumo A.Y., Peres-da-Silva A., Broz D.K., Baum R., Guo S., Chen J.K., Attardi L.D, & Chang H.Y. (2016). An inducible long noncoding RNA amplifies DNA damage signaling. Nature genetics, 48(11), 1370-1376.
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2

Knockdown of DINO in Lung Fibroblasts

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human fetal lung fibroblasts were transfected with 3 independent 50 nM ON-TARGETPlus siRNAs (Dharmacon) or 100nM ASOs40 (link) targeting DINO using the Lipofectamine 2000 reagent. Large scale transfections were performed using the Amaxa Nucleofector NHDF kit. siRNAs for mRNAs (Ambion) were used as a pool of two. U2OS cells were infected with shRNAs targeting DINO using the pGIPZ shRNAmir lentiviral system.
Schmitt A.M., Garcia J.T., Hung T., Flynn R.A., Shen Y., Qu K., Payumo A.Y., Peres-da-Silva A., Broz D.K., Baum R., Guo S., Chen J.K., Attardi L.D, & Chang H.Y. (2016). An inducible long noncoding RNA amplifies DNA damage signaling. Nature genetics, 48(11), 1370-1376.
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