Anti msh2

IHC staining was performed on formalin-fixed paraffin-embedded sections (4 µm) according to a standard protocol. Briefly, sections were deparaffinized with xylene twice for 10 min and rehydrated with 100% ethanol twice for 5 min, 95% ethanol twice for 2 min and 85% ethanol for 2 min, then washed in deionized H₂O for 1 min at room temperature with stirring. Subsequently, sections were treated with 3% H₂O₂ at room temperature for 10 min and subjected to antigen retrieval by citrate buffer (pH 6.0) at 98°C for 15 min. Following blocking with 5% bovine serum albumin for 20 min at room temperature, the sections were incubated at 4°C overnight with anti-USP10 (1:250; cat. no. ab109219; Abcam, Cambridge, UK) and anti-MSH2, (cat. no. ZA-0622; ready-to-use; OriGene Technologies, Inc., Beijing, China) primary antibodies. The sections were incubated with biotinylated antibodies and horseradish peroxidase-labeled streptavidin [Ready-to-use; UltraSensitive™ SP (Mouse/Rabbit) IHC kit-9710; Fuzhou Maixin Biotech Co., Ltd., China] for 15 min each at room temperature. The reaction products were visualized with 3,3′-diaminobenzidine as a chromogen followed by counterstaining with hematoxylin at room temperature for 1 min. The sections without primary antibody served as a negative control (18 (link)).