The adherent cancer cell lines HeLa, HEK293, A549, and HaCaT were obtained from NCCS, Pune. All cells were cultured in high glucose DMEM media (D1152, Sigma-Aldrich) containing 10% FBS and 5% CO2. We followed the standard protocol50 (link) for the western blotting of the lysates from the mentioned cell lines. Cadherin-23 (HPA017232, Sigma-Aldrich and PA5-43398, Invitrogen), eGFP (A11122, Invitrogen), and His-tag (11965085001, Roche) antibodies were used at the concentration of 1 μg/ml to detect the proteins.
RNA from different cancer cell lines was extracted using RNA isolation kit (Bio Rad) and treated with DNAse using DNAse 1 kit (AMPD1, Sigma-Aldrich). cDNA synthesis was done using a cDNA synthesis kit (Bio Rad). qRT-PCR was performed with the primers probing Cdh23 using the real-time PCR system (CFX96 Bio Rad).
RNA from different cancer cell lines was extracted using RNA isolation kit (Bio Rad) and treated with DNAse using DNAse 1 kit (AMPD1, Sigma-Aldrich). cDNA synthesis was done using a cDNA synthesis kit (Bio Rad). qRT-PCR was performed with the primers probing Cdh23 using the real-time PCR system (CFX96 Bio Rad).