Flow cytometric analysis of total mouse splenocytes or bone marrow cells was performed using the following antibodies: B220-BV605 (RA3-6B2), CD4-AF700 (RMP4-5), CD44-APC (IM7), CD62L-PECy7 (MEK-14), PD1-PE (29F.1A12), IgM-BV605 (RMM-1), IgD-BV711 (11-26c2a), CD93-PE (AA4.1), Streptavidin-PECy5, MHCII-PECy7 (M5/114.15.2), Ly51-biotin (6C3), CD24-APC (M1/69), CD23-biotin (B3H4). GL7-FITC (GL-7), CD95- PeCy7, CXCR5-biotin (2G8), CD43-FITC (S7), CD19-biotin (1D3), CD90.2-biotin (53-2.1), CD11b-AF700 (M1/70), CD11c-FITC (HL3) (BD Biosciences). CD8α (clone 53–6.7) (eBioscience). All cells were stained with fixable viability dye-eFluor780 (Invitrogen) prior to surface staining. Stained cells were analyzed using the BD LSR II flow cytometer (BD Biosciences). Data were acquired using FACSDiva software (BD Biosciences) and analyzed using FlowJo software (Tree Star).
Cd11c fitc hl3
Manufactured by BD
CD11c-FITC (HL3) is a fluorescently labeled antibody that binds to the CD11c antigen. CD11c is a cell surface marker expressed on dendritic cells and some subsets of macrophages and monocytes. The FITC (fluorescein isothiocyanate) label allows for the detection and identification of cells expressing CD11c.
Automatically generated - may contain errors
Lab products found in correlation
Cd43 fitc, by BD (1 mentions)
Cd8α clone 53 6, by Thermo Fisher Scientific (1 mentions)
Cd19 biotin, by BD (1 mentions)
Cd11b af700, by BD (1 mentions)
Efluor 780, by Thermo Fisher Scientific (1 mentions)
Facsdiva software, by BD (1 mentions)
Lsr 2 flow cytometer, by BD (1 mentions)
Micropestle, by Eppendorf (1 mentions)
Cd19 pe 6d5, by BioLegend (1 mentions)
Collagenase 2, by Thermo Fisher Scientific (1 mentions)
Cd3 apc 17a2, by Thermo Fisher Scientific (1 mentions)
Accuri c6 flow cytometer, by BD (1 mentions)
2 protocols using cd11c fitc hl3
1
Comprehensive Mouse Immune Cell Analysis
2
Cornea and Lymph Node Immune Cell Isolation
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Eyes were harvested, and corneas were isolated under a dissecting microscope at various times postinfection. Corneas were digested in 400 U/ml collagenase II (Life Technologies) for 1.5 h at 37°C, homogenized with an Eppendorf micropestle, triturated with a pipettor, and filtered through a 70-µm mesh filter before flow cytometry staining. Cervical lymph nodes were dissected, similarly homogenized, and filtered before flow cytometry staining. Blood was harvested by submandibular bleed, and erythrocytes (RBCs) were lysed using Gey’s solution. The following antibodies were used for flow cytometric analysis: Ly6G-fluorescein isothiocyanate (FITC) (1A6), CD4-FITC (GK1.5), CD69-phycoerythrin (PE) (H1.2F3), CD8α-PE (53-6.7), CD11c-FITC (HL3), and GR1-peridinin chlorophyll protein (PerCP)/Cy5.5 (RB6-8C5), all purchased from BD Biosciences (San Jose, CA). Ly6C-PerCP/Cy5.5 (HK1.4), CD11b-allophycocyanin (APC) (M1/70), CD3-PerCP/Cy5.5 (17A2), CD45-APC (30-F11), and CD19-PE (6D5) were purchased from BioLegend (San Diego, CA), and CD3-APC (17A2) was purchased from eBioscience (San Diego, CA). Samples were analyzed using the BD Accuri C6 flow cytometer, and the data were analyzed using CFlow software (Accuri).
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