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3 protocols using potassium persulfate

1

Potassium Persulfate Oxidation Protocol

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Potassium persulfate was purchased from Junsei Chemical (Tokyo, Japan). Pyrocatechol violet (PV) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). All other chemicals were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA).
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2

Antioxidant Activity Evaluation Protocol

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Vanillyl alcohol (4-hydroxy-3-methoxybenzyl alcohol, VA), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,6-di-tert-butyl-4-methylphenol (BHT), 2,2'-azino-bis (3-ethylbenzothiazoline-6sulphonic acid) (ABTS), Menhaden oil (MO), bacterial propidium iodide (PI) solution, and 1butanol were purchased from Sigma-Aldrich Co. (USA). Toluene, acetone, and potassium persulfate were purchased from Junsei Co. (Japan), while methanol was purchased from Merck Co. (Germany). Linseed oil, methanol and HPLC grade water were purchased from Samchun Chemicals Co. (Korea). LB broth was purchased from Becton, Dickinson and Co.
(USA). Candida antartica lipase (CalB) immobilized on acrylic resin was purchased from Sigma-Aldrich Co. (USA).
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3

Antioxidant and Cytotoxicity Evaluation

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The following chemicals were procured from Sigma-Aldrich, St. Louis, MO, USA: ascorbic acid, 1,1-diphenyl,2-picryl hydrazyl (DPPH), gallic acid, vanillin, (+)-catechin, sulfuric acid, sodium dodecyl sulphate (SDS), sulfuric acid (H2SO4), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, potassium ferricyanide (K3[Fe(CN)6]), ferrous sulphate (FeSO4), ferric chloride (FeCl3), sodium carbonate (Na2CO3), sodium nitrite (NaNO2), sodium hydroxide (NaOH), aluminum chloride (AlCl3), copper(II) chloride (CuCl2), iron(II) chloride (FeCl2), ethanolic neocuproine, Folin–Ciocalteu's phenol reagent, 2,2′-bipyridyl, ethylenediaminetetraacetic acid (EDTA), ammonium acetate, dimethyl sulfoxide (DMSO), and propidium iodide (PI). Potassium persulfate (Junsei, Japan), HPLC grade methanol, and ethanol (J.T Baker, U.S.A) were the other chemicals used.
RPMI 1640 medium was purchased from Thermo SCIENTIFIC, DMEM from Gendepot, and the cell counting Kit-8 (CCK-8) from Dojindo Laboratories.
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