Medium and supplements for cell culture were purchased from Biochrom AG (Berlin, Germany) or GIBCO Invitrogen (Karlsruhe, Germany). All reagents (e.g., Ascorbic acid A4403; Trypan Blue T8154), except those specified below, were purchased from Sigma-Aldrich (Deisenhofen, Germany). Capsazepine was purchased from Cayman Chemical Company (Ann Arbor, Mi, USA). The internal and external solutions for planar patch-clamping were provided by Nanion Company (Munich, Germany). Fura2/AM was purchased from PromoCell (Heidelberg, Germany).
Fura2 am
Manufactured by PromoCell
Sourced in Germany
Fura2/AM is a fluorescent calcium indicator used for measuring intracellular calcium levels. It is a cell-permeable, ratiometric dye that changes its fluorescence properties upon binding to calcium ions. Fura2/AM can be used with a variety of cell types and experimental setups to monitor calcium dynamics.
Automatically generated - may contain errors
2 protocols using fura2 am
1
Cell Culture and Patch-Clamp Assays
2
Cell Culture Reagents and Sourcing
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Cell culture medium and other cell culture supplements were purchased from Biochrom AG (Berlin, Germany) or GIBCO Invitrogen (Karlsruhe, Germany). All reagents (e.g., trypan blue T8154) were purchased from Sigma-Aldrich (Diesenhofen, Germany) unless otherwise specified. Recombinant Human β-NGF was purchased from Biogems company (Westlake Village, CA, USA). Icilin, AM251, and WIN55,212-2 were purchased from Cayman Chemical Company (Ann Arbor, MI, USA). For calcium imaging, the fluorescent dye fura-2/AM was purchased from PromoCell GmbH (Heidelberg, Germany).
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